The regulation of the Drosophila msl-2 gene reveals a function for Sex-lethal in translational control
The regulation of the Drosophila msl-2 gene reveals a function for Sex-lethal in translational control
复制标题
DOI：
10.1016/s0092-8674(00)80262-7
复制
发表时间：
1997-05-30
期刊：
影响因子：
64.5
通讯作者：
Baker, BS
中科院分区：
文献类型：
--
作者：
Bashaw, GJ;Baker, BS
在果蝇中，剂量补偿通过增加单个雄性X染色体的转录而发生。这一过程需要四个由雄性特异性致死基因编码的反式作用因子。剂量补偿通过剪接调节剂性致死（Sex-lethal）限制于雄性，其功能是通过未知机制阻止雌性中MSL-2蛋白的产生。在这份报告中，我们提供的证据表明，性致死的行为协同作用，通过在MSL-2的5'和3'非翻译区的序列介导的镇压。我们还提供了证据表明，MSL-2的抑制是直接调控性致死在翻译水平。
In Drosophila, dosage compensation occurs by increasing the transcription of the single male X chromosome. Four trans-acting factors encoded by the male-specific lethal genes are required for this process. Dosage compensation is restricted to males by the splicing regulator Sex-lethal, which functions to prevent the production of the MSL-2 protein in females by an unknown mechanism. In this report, we provide evidence that Sex-lethal acts synergistically through sequences in both the 5' and 3' untranslated regions of MSL-2 to mediate repression. We also provide evidence that the repression of MSL-2 is directly regulated by Sex-lethal at the level of translation.