Pigment accumulation and transcription of LhMYB12 and anthocyanin biosynthesis genes during flower development in the Asiatic hybrid lily (Lilium spp.)
Pigment accumulation and transcription of LhMYB12 and anthocyanin biosynthesis genes during flower development in the Asiatic hybrid lily (Lilium spp.)
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DOI：
10.1016/j.plantsci.2012.05.013
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发表时间：
2012-09-01
期刊：
影响因子：
5.2
通讯作者：
Yamagishi, Masumi
中科院分区：
文献类型：
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作者：
Lai, Yun-Song;Shimoyamada, Yoshihiro;Yamagishi, Masumi
花色素苷的生物合成通常受MYB转录因子的调控，MYB转录因子分为AN 2和C1亚类。AN 2亚群调控真双子叶植物花色素苷合成途径中的晚期基因，而C1亚群控制单子叶植物中的早期和晚期基因。花色苷是亚洲百合的主要色素，其中LhMYB 12是单子叶植物中的第一个AN 2亚群。在这项研究中，在花芽发育过程中的色素和基因转录的积累进行了评估，以确定LhMYB 12调控的基因。LhMYB 12和花青素合成基因表现出相同的转录谱，LhMYB 12直接激活查尔酮合成酶和二氢黄酮醇4-还原酶的启动子。这表明LhMYB 12调节早期和晚期基因，尽管属于AN 2亚组。品种Landini积累花色素苷和黄酮醇。这些色素的含量在花芽发育后期增加，这可能是早期和晚期基因协调表达的结果。在花芽发育的早期阶段，花被片不含黄酮类化合物，但积累了肉桂酸衍生物。这些结果表明，百合花被片色素积累和基因转录在被子植物花中是独特的。(C)2012爱思唯尔爱尔兰有限公司保留所有权利。
Anthocyanin biosynthesis is often regulated by MYB transcription factors that are classified into AN2 and C1 subgroups. The AN2 subgroup regulates the late genes in the anthocyanin biosynthesis pathway of eudicots, whereas the C1 subgroup controls both early and late genes in monocots. Anthocyanin is a major pigment in Asiatic hybrid lilies (Lilium spp.), with LhMYB12 being the first AN2 subgroup in monocots. In this study, the accumulation of pigments and gene transcripts during flower bud development was evaluated to determine the genes regulated by LhMYB12. LhMYB12 and anthocyanin biosynthesis genes showed the same transcription profiles, with LhMYB12 directly activating the promoters of chalcone synthase and dihydroflavonol 4-reductase. This indicates that LhMYB12 regulates both early and late genes, despite belonging to the AN2 subgroup. The cultivar Landini accumulated anthocyanin and flavonol. The contents of these pigments increased during the late stages of flower bud development; this might result from the coordinated expression of early and late genes. During the early stages of flower bud development, the tepals contained no flavonoids but accumulated cinnamic acid derivatives. These results indicate that the profiles of pigment accumulation and gene transcription in lily tepals are unique among angiosperm flowers. (C) 2012 Elsevier Ireland Ltd. All rights reserved.