The response of testicular leukocytes to lipopolysaccharide-induced inflammation: further evidence for heterogeneity of the testicular macrophage population
The response of testicular leukocytes to lipopolysaccharide-induced inflammation: further evidence for heterogeneity of the testicular macrophage population
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DOI：
10.1007/s00441-002-0547-6
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发表时间：
2002-05-01
影响因子：
3.6
通讯作者：
Hedger, MP
中科院分区：
文献类型：
--
作者：
Gerdprasert, O;O'Bryan, MK;Hedger, MP
大鼠睾丸中的大多数巨噬细胞可以通过组织驻留巨噬细胞标志物ED 2来识别。睾丸内巨噬细胞的较小群体不表达ED 2抗原，但对单核细胞/巨噬细胞标志物ED 1呈阳性。用炎症刺激物脂多糖（LPS）处理成年大鼠对睾丸驻留（ED 2（+））巨噬细胞的数量没有影响，但引起ED 1（+）ED 2（-）单核细胞样巨噬细胞的短暂增加（12小时后平均增加3倍）。在对照组和LPS处理的大鼠睾丸中，大多数表达ED 1的巨噬细胞和所有间质细胞对一氧化氮合酶（iNOS）的诱导型亚型呈免疫阳性。然而，即使在LPS处理后，也只有不到6%的ED 2（+）巨噬细胞显示出任何iNOS表达。这一缺陷通过发现在体外用LPS刺激的分离的ED 2（+）睾丸巨噬细胞（>98%纯度）不产生NO而得到证实。与此相反，居民从腹膜巨噬细胞显示预期的NO反应，和纯化的Leydig细胞产生显着的NO，无论是否存在的LPS。总之，这些数据表明成年大鼠睾丸中存在至少两种巨噬细胞亚群：（1）ED 2（+）驻留巨噬细胞，其在LPS处理后不改变，并且大多数不表达iNOS或响应于炎性刺激产生NO，和（2）ED 1（+）ED 2（-）单核细胞样巨噬细胞，其在LPS处理后数量增加，并且即使在没有外源性炎症刺激的情况下也表达iNOS。很可能这些不同的亚群在睾丸内具有不同的功能作用。
The majority of macrophages in the rat testis can be identified by the tissue-resident macrophage marker ED2. A smaller population of intratesticular macrophages do not express the ED2 antigen but are positive for the monocyte/macrophage marker ED1. Treatment of adult rats with the inflammatory stimulus lipopolysaccharide (LPS) had no effect on the number of testicular resident (ED2(+)) macrophages but caused a transient increase in ED1(+)ED2(-) monocyte-like macrophages (an average three-fold increase 12 h later). In both control and LPS-treated rat testes, a majority of macrophages that expressed ED1 and all Leydig cells were immuno-positive for the inducible isoform of nitric oxide synthase (iNOS). However, less than 6% of ED2(+) macrophages showed any iNOS expression, even after LPS treatment. This deficiency was confirmed by the finding that isolated ED2(+) testicular macrophages (>98% pure) stimulated with LPS did not produce NO in vitro. In contrast, resident macrophages from the peritoneum showed the expected NO response, and purified Leydig cells produced significant NO regardless of the presence or absence of LPS. Collectively, these data indicate the presence of at least two macrophage subsets in the adult rat testis: (1) the ED2(+) resident macrophages, which do not alter following LPS-treatment and mostly do not express iNOS or produce NO in response to an inflammatory stimulus, and (2) the ED1(+)ED2(-) monocyte-like macrophages, which increase in number after LPS-treatment and express iNOS even in the absence of exogenous inflammatory stimulation. It is highly probable that these different subsets have different functional roles within the testis.