IN-VIVO EVALUATION OF THE PATHOGENICITY OF FIELD ISOLATES OF INFECTIOUS-BRONCHITIS VIRUS
IN-VIVO EVALUATION OF THE PATHOGENICITY OF FIELD ISOLATES OF INFECTIOUS-BRONCHITIS VIRUS
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DOI：
10.2307/1592083
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发表时间：
1994-07-01
期刊：
影响因子：
1.4
通讯作者：
KING, DJ
中科院分区：
文献类型：
--
作者：
AVELLANEDA, GE;VILLEGAS, P;KING, DJ
采用鸡传染性支气管炎病毒（IBV）与大肠杆菌混合感染模型，对1989 ~ 1992年从格鲁吉亚肉鸡场分离的13株IBV进行了致病性测定。 根据临床症状、死亡率和病变程度，将分离株分为高致病性、中等致病性和低致病性。 将体内分型结果与逆转录-聚合酶链反应-限制性片段长度多态性分析的血清型分型结果进行比较。 高致病性组由代表三种血清型的五个分离株组成：阿肯色州、格鲁吉亚变异株（GAV）和马萨诸塞州。 中、低致病性组的分离株除1株属于马萨诸塞州血清型外，均为康涅狄格州血清型的代表。
The pathogenicity of 13 field isolates of infectious bronchitis virus (IBV) isolated from Georgia broiler farms from 1989 to 1992 was evaluated using the IBV and Escherichia coli mixed-infection model. Based on the clinical signs, mortality, and lesions, the isolates were classified as high, intermediate, and low in pathogenicity. The in vivo classification was compared with the serotype classification results obtained by reverse transcriptase-polymerase chain reaction-restriction fragment length polymorphism analysis. The high-pathogenicity group was composed of five isolates representing three serotypes: Arkansas, Georgia variant (GAV), and Massachusetts. Isolates in the intermediate- and low-pathogenicity groups were all representatives of the Connecticut serotype, except for one isolate, which belonged to the Massachusetts serotype.