Expression and functional analysis of FaPH01;H9 gene of strawberry (Fragariaxananassa)
Expression and functional analysis of FaPH01;H9 gene of strawberry (Fragariaxananassa)
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草莓FaPH01;H9基因的表达及功能分析
DOI：
10.1016/s2095-3119(16)61433-8
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发表时间：
2017-01-01
影响因子：
4.8
通讯作者：
Zhang Zhi-hong
中科院分区：
文献类型：
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作者：
Cao Fei;Li He;Zhang Zhi-hong
虽然磷酸盐1（PH 01）基因家族与无机磷转运和体内平衡有关，但该基因在草莓中的潜在机制尚未被揭示。在本研究中，我们分析了PH 01;H9基因在草莓（Fragariaxananassa）中的表达，揭示了该基因参与磷（P）含量的调节。PH 01;H9基因的编码序列（CDS）从栽培草莓'Sachinoka'中分离并命名为FaPH 01;H9。该基因的CDS全长为2 292 bp，编码763个氨基酸，含有SYG 1/Pho 81/XPR 1（SPX）和ERD 1/XPR 1/SYG 1（EXS）结构域，参与磷酸（Pi）信号转导。实时荧光定量RT-PCR结果表明，除叶柄外，FaPH 01;H9基因在不同器官中的表达量与磷含量基本一致。不同发育时期果实中的P含量与其表达量也有一定的相关性。在不同浓度磷肥处理下，FaPH 01;H9的表达量与叶片中磷含量的变化趋势一致。此外，获得了转基因拟南芥品系，并且过表达FaPH 01;H9的拟南芥植株中的P含量显著高于野生型植株。因此，我们认为FaPHO 1;H9在磷转运中起作用。
Although the phosphate 1 (PH01) gene family has been implicated in inorganic phosphate transport and homeostasis, the underlying mechanism of this gene in the strawberry has not yet been revealed. In the present study, we analyzed the expression of the PH01;H9 gene in the strawberry (Fragariaxananassa), revealing the involvement of this gene in the regulation of phosphorus (P) content. The coding sequence (CDS) of the PH01;H9 gene, was isolated from the cultivated strawberry 'Sachinoka' and named as FaPH01;H9. The full-length CDS of this gene was 2 292 bp, encoding 763 amino acids, and the protein contained both SYG1/Pho81/XPR1 (SPX) and ERD1/ XPR1/SYG1 (EXS) domains, which were involved in phosphate (Pi) signaling. Real-time reverse transcription-polymerase chain reaction-(RT-PCR) data suggested that the level of FaPH01;H9 expression was consitent with the P content in different organs, except for the petiole. Particularly, its expression level was also correlated with P content in fruits of different developmental stages. The expression of FaPH01;H9 was also consistent with P content in leaves under different concentrations of P fertilizer application. Furthermore, transgenic Arabidopsis lines were generated, and the P content in Arabidopsis plants over-expressing FaPH01;H9 was significantly higher than that in wild-type plants. Therefore, we proposed that FaPHO1;H9 functions in P transport.