High-throughput phenotyping of uropathogenic E. coli isolates with Fourier transform infrared spectroscopy
High-throughput phenotyping of uropathogenic E. coli isolates with Fourier transform infrared spectroscopy
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DOI：
10.1039/c3an36517d
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发表时间：
2013-01-01
期刊：
影响因子：
4.2
通讯作者：
Goodacre, Royston
中科院分区：
文献类型：
--
作者：
AlRabiah, Haitham;Correa, Elon;Goodacre, Royston
傅里叶变换红外 (FT-IR) 光谱是一种成熟的快速整体生物指纹识别方法，可生成反映所研究微生物表型的细菌代谢指纹。然而，虽然 FT-IR 光谱速度很快（每个样品通常需要 10 秒到 1 分钟），但微生物样品制备方法可能非常耗时，因为微生物的生长使用平板培养或摇瓶。我们报告了一种新方法，可以将小体积（通常为 200 μL）的细菌微培养与 FT-IR 光谱相结合。这种方法快速且易于执行，并为更长且更昂贵的摇瓶培养（样品体积 20 mL）提供相同的数据。通过这种微培养方法，我们还证明了代谢指纹的高再现性。该方法可以分离涉及尿路感染的不同大肠杆菌菌株，包括全球传播的 ST131 克隆的成员，包括基因型和对抗生素环丙沙星的耐药性或其他方面。
Fourier transform infrared (FT-IR) spectroscopy is an established rapid whole-organism fingerprinting method that generates metabolic fingerprints from bacteria that reflect the phenotype of the microorganism under investigation. However, whilst FT-IR spectroscopy is fast (typically 10 s to 1 min per sample), the approaches for microbial sample preparation can be time consuming as plate culture or shake flasks are used for growth of the organism. We report a new approach that allows microcultivation of bacteria from low volumes (typically 200 mu L) to be coupled with FT-IR spectroscopy. This approach is fast and easy to perform and gives equivalent data to the lengthier and more expensive shake flask cultivations (sample volume 20 mL). With this micro-culture approach we also demonstrate high reproducibility of the metabolic fingerprints. The approach allowed separation of different isolates of Escherichia coli involved in urinary tract infection, including members of the globally disseminated ST131 clone, with respect to both genotype and resistance or otherwise to the antibiotic Ciprofloxacin.