Formation of a keratin layer with silk fibroin-polyethylene glycol composite hydrogel fabricated by digital light processing 3D printing
Formation of a keratin layer with silk fibroin-polyethylene glycol composite hydrogel fabricated by digital light processing 3D printing
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DOI：
10.1016/j.jiec.2018.12.023
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发表时间：
2019-04-25
影响因子：
6.1
通讯作者：
Hyun, Jinho
中科院分区：
文献类型：
--
作者：
Kwak, Hojung;Shin, Sungchul;Hyun, Jinho
将丝素蛋白（SF）掺入的4-臂聚乙二醇丙烯酸酯（PEG 4A）用作用于数字光处理三维打印的生物油墨。该过程采用可见光范围，以确保生物墨水中细胞的安全性。将成纤维细胞包埋在打印的水凝胶中，并在水凝胶的表面培养角质形成细胞。使用生物墨水材料PEG 4A和SF-PEG 4A的细胞增殖以及这些材料的毒性通过活/死和alamarBlue测定来验证。SF-PEG 4A水凝胶支架上的原代人角质形成细胞通过组织培养在气液界面产生角蛋白层，促进成熟和分层。SF聚乙二醇水凝胶显示出更高的细胞增殖和细胞铺展，与PEG 4A水凝胶相比，SF-PEG 4A水凝胶形成更厚的角蛋白层。(C)2018年韩国工业与工程化学学会。Elsevier B. V.出版，保留所有权利。
Silk fibroin (SF)-incorporated 4-arm polyethylene glycol acrylate (PEG4A) was used as a bioink for digital light processing three-dimensional printing. A visible range of light was adopted for the process to assure the safety of the cells in the bioink. Fibroblast cells were embedded in the printed hydrogels, and the keratinocyte cells were cultured at the surfaces of the hydrogels. The proliferation of cells with the bioink materials, PEG4A and SF-PEG4A, and the toxicity of these materials, were verified by live/dead and alamarBlue assays. The primary human keratinocytes on the SF-PEG4A hydrogel scaffold generated a keratin layer by the tissue culture at the air-liquid interface, promoting maturation and stratification. SF polyethylene glycol hydrogels showed higher cell proliferation and cell spreading, and the thicker keratin layer was formed with SF-PEG4A hydrogels compared with PEG4A hydrogels. (C) 2018 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.