BDK knockout skeletal muscle satellite cells exhibit enhanced protein translation initiation signal in response to BCAA in vitro.
BDK knockout skeletal muscle satellite cells exhibit enhanced protein translation initiation signal in response to BCAA in vitro.
复制标题
BDK 敲除骨骼肌卫星细胞在体外响应 BCAA 表现出增强的蛋白质翻译起始信号。
DOI：
10.1093/bbb/zbac021
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Higashida K.
中科院分区：
文献类型：
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作者：
Nakai N;Iida N;Kitai S;Shimomura Y;Kitaura Y;Higashida K.
我们研究了支链氨基酸（BCAAs）和电脉冲刺激（EPS）对从支链α-酮酸脱氢酶激酶（BDK）敲除（KO）小鼠分离的肌卫星细胞（MSCs）mTORC 1通路的影响。从BDK KO和野生型（WT）小鼠中分离MSC，增殖并分化成肌管。BCAA刺激增加了来自WT和BDK KO小鼠的MSC中p70 S6激酶（p70 S6 K）的磷酸化，p70 S6 K是蛋白质翻译起始的标志物，但从BDK KO小鼠分离的MSC中增加的速率更高。因此，EPS对p70 S6 K磷酸化的增加没有差异。使用shRNA在WT小鼠的MSC中的急性BDK敲低降低了对BCAA刺激的响应的p70 S6 K磷酸化。总的来说，mTORC 1对BCAA刺激的敏感性通过BCAA催化剂的慢性而非急性增强而升高。
We examined the effects of branched-chain amino acids (BCAAs) and electrical pulse stimulation (EPS) on the mTORC1 pathway in muscle satellite cells (MSCs) isolated from branched-chain α-keto acid dehydrogenase kinase (BDK) knockout (KO) micein vitro. MSCs were isolated from BDK KO and wild-type (WT) mice, proliferated, and differentiated into myotubes. BCAA stimulation increased the phosphorylation of p70 S6 kinase (p70S6K), a marker of protein translation initiation, in MSCs from WT and BDK KO mice, but the rate of the increase was higher in MSCs isolated from BDK KO mice. Contrarily, there was no difference in the increase in p70S6K phosphorylation by EPS. Acute BDK knockdown in MSCs from WT mice using shRNA decreased p70S6K phosphorylation in response to BCAA stimulation. Collectively, the susceptibility of mTORC1 to BCAA stimulation was elevated by chronic, but not acute, enhancement of BCAA catabolism.