Genetic analysis of Escherichia coli biofilm formation:: roles of flagella, motility, chemotaxis and type I pili
Genetic analysis of Escherichia coli biofilm formation:: roles of flagella, motility, chemotaxis and type I pili
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DOI：
10.1046/j.1365-2958.1998.01061.x
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发表时间：
1998-10-01
影响因子：
3.6
通讯作者：
Kolter, R
中科院分区：
文献类型：
--
作者：
Pratt, LA;Kolter, R
我们使用大肠杆菌作为模型系统来研究生物膜形成的起始。在这里，我们证明了大肠杆菌在多种非生物表面上以一种依赖于营养的方式形成生物膜。此外，我们还分离出了插入突变，这些突变使这种有机体在生物膜形成方面存在缺陷。这些突变中有一半被发现扰乱了正常的鞭毛功能。使用已定义的fli、flh、mot和ChE等位基因，我们证明了运动性而不是趋化性对正常生物膜的形成至关重要。对这些突变体的微观分析表明，运动性对于与表面的初始相互作用和沿表面的运动都是重要的。此外，我们提出的证据表明，I型菌毛(含有甘露糖特异的粘附素，FimH)是初始表面附着所必需的，甘露糖抑制了正常的附着。根据这里提出的观察结果，讨论了一个描述运动性和I型菌毛在生物膜发育中的作用的工作模型。
We have used Escherichia coli as a model system to investigate the initiation of biofilm formation. Here, we demonstrate that E. coliforms biofilms on multiple abiotic surfaces in a nutrient-dependent fashion. In addition, we have isolated insertion mutations that render this organism defective in biofilm formation. one-half of these mutations was found to perturb normal flagellar function. Using defined fli, flh, mot and che alleles, we show that motility, but not chemotaxis, is critical for normal biofilm formation. Microscopic analyses of these mutants suggest that motility is important for both initial interaction with the surface and for movement along the surface. In addition, we present evidence that type I pili (harbouring the mannose-specific adhesin, FimH) are required for initial surface attachment and that mannose inhibits normal attachment. In light of the observations presented here, a working model is discussed that describes the roles of both motility and type I pili in biofilm development.