Effect of Sonication on Plant Stomatal Movement
Effect of Sonication on Plant Stomatal Movement
复制标题
DOI：
10.32473/ufjur.v22i0.121769
复制
发表时间：
2020-11
期刊：
影响因子：
--
通讯作者：
Suren Jeevaratnam;Chu-Yund Lin;Sixue Chen
中科院分区：
文献类型：
--
作者：
Suren Jeevaratnam;Chu-Yund Lin;Sixue Chen
本研究的主要目标是确定超声处理对气孔运动的影响。一个次要目标是确定超声处理最有效去除叶肉细胞和富集保卫细胞的最佳时间间隔。在本研究中，拟南芥的远轴叶皮以设定的振幅超声处理 1、3、5 和 7 分钟的时间间隔，以分析叶肉细胞的去除情况。为了并置叶子并确定保卫细胞富集，在超声处理之前和之后拍摄显微图像。此外，为了确定气孔是活的，中性红染色与 40 倍放大倍数结合使用。据推测，超声处理是去除叶肉细胞和富集保卫细胞的有效方法。这项研究的结果表明，超声处理实际上是保卫细胞富集的有效方案；然而，它对于保卫细胞纯化并不那么有效。这是由于超声处理后存在叶肉细胞和表皮层。先前有关超声处理的研究非常普遍。然而，用于去除拟南芥叶肉细胞的超声处理的研究尚未得到广泛研究。因此，无法获得支持这项研究的先前信息。然后将实验第一部分的结果进行扩展，以确定超声处理如何影响气孔运动。确定实验组的平均气孔孔径在两小时内下降。
The primary goal of this study was to determine the effect of sonication on stomatal movement. A minor goal was to determine the best time interval at which sonication is the most effective at removing mesophyll cells and enriching guard cells. For this study, abaxial leaf peels of Arabidopsis thaliana were sonicated for 1, 3, 5, and 7-minute intervals at a set amplitude to analyze the removal of mesophyll cells. To juxtapose the leaves and to determine guard cell enrichment, microscopic images were taken prior to and after sonication. Furthermore, to establish that the stomata are alive, neutral red staining was used in conjunct with 40x magnification. It was hypothesized that sonication is an effective method for the removal of mesophyll cells and the enrichment of guard cells. The results of this study suggest that sonication is in fact an effective protocol for guard cell enrichment; however, it is not as effective for guard cell purification. This is due to the presence of mesophyll cells and epidermal layers present after sonication. Previous research dealing with sonication is very prevalent; however, research on sonication dealing with the removal of mesophyll cells in Arabidopsis thaliana is not widely studied. Thus, previous information to support this study could not be attained. Results from the first part of the experiment were then extended to determine how sonication affects stomatal movement. It was determined that in the experimental group, the average stomatal aperture decreased over a two-hour period.