Variability of hydrocarbon and fatty acid components in cultures of the filamentous cyanobacterium Scytonema sp isolated from microbial community "Black Cover" of limestone walls in Jerusalem

Variability of hydrocarbon and fatty acid components in cultures of the filamentous cyanobacterium Scytonema sp isolated from microbial community "Black Cover" of limestone walls in Jerusalem
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DOI:
10.1023/a:1021309623541
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发表时间:
2002-11-01
影响因子:
2.8
通讯作者:
Srebnik, M
Srebnik, M
中科院分区:
生物学4区
文献类型:
--
作者:
Dembitsky, VM;Srebnik, M

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丝状蓝藻 Scytonema sp. 四种菌株的碳氢化合物和脂质成分。使用串联毛细管柱通过气相色谱-质谱法鉴定了从耶路撒冷石灰岩墙壁的微生物群落“黑盖”中分离出来的细菌。主要化合物为:1-十七炔 (1.5-8%)、十六烷酸 (14-36%)、(Z,Z)-9,12-十八碳二烯酸 (12-30%)、(Z,Z,Z)-9,12,15-十八碳三烯酸 (6-12%)、正十七烷 (4-16%) 和1-十七碳烯(1.5-8%)。除了不饱和烷烃和脂肪酸外,还分离出极长链 (C-30-C-32) 烃、角鲨烯 (2.4-3.0%) 和支链 4,8,12-三甲基-C-13:0 酸。使用 GC-MS 和 C-13-NMR 在蓝藻物种中检测到两种主要碳氢化合物。
The hydrocarbon and lipid components of four strains of the filamentous cyanobacterium Scytonema sp. isolated from microbial community "Black Cover" of limestone walls in Jerusalem were identified by gas chromatography-mass spectrometry using serially coupled capillary columns. The dominant compounds were: 1-heptadecyne (1.5-8%), hexadecanoic acid (14-36%), (Z,Z)-9,12-octadecadienoic acid (12-30%), (Z,Z,Z)-9,12,15-octadecatrienoic acid (6-12%), n-heptadecane (4-16%), and 1-heptadecene (1.5-8%). In addition to unsaturated alkanes and fatty acids, the very long-chain (C-30-C-32) hydrocarbons, squalene (2.4-3.0%), and branched 4,8,12-trimethyl-C-13:0 acid were also isolated. Two major hydrocarbons were detected in the cyanobacteria species using GC-MS and C-13-NMR.