Dextran coated ultrafine superparamagnetic iron oxide nanoparticles: compatibility with common fluorometric and colorimetric dyes.
Dextran coated ultrafine superparamagnetic iron oxide nanoparticles: compatibility with common fluorometric and colorimetric dyes.
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DOI：
10.1021/ac200103x
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发表时间：
2011-05-15
影响因子：
7.4
通讯作者：
Doak, Shareen H.
中科院分区：
文献类型：
--
作者：
Griffiths, Sioned M.;Singh, Neenu;Jenkins, Gareth J. S.;Williams, Paul M.;Orbaek, Alvin W.;Barron, Andrew R.;Wright, Chris J.;Doak, Shareen H.
由于纳米材料（NM）独特的物理化学性质及其未知的反应性，NM改变用于测量其细胞毒性和遗传毒性的荧光/比色探针的光学性质的可能性可能导致读数不准确。这可能具有潜在的影响，因为NM，如超细超顺磁性氧化铁纳米颗粒（USPION），越来越多地发现它们在纳米医学中的用途，并且基于吸光度/荧光的测定用于评估它们的毒性。本研究考察了葡聚糖包被的USPION（dUSPION）（磁赤铁矿和磁铁矿）改变用于评价细胞毒性（MTS、CyQUANT、钙黄绿素和EthD-1）和氧化应激（DCFH-DA和APF）的常用探针背景信号的潜力。在本研究中，两种形式的dUSPION均引起MTS信号增加，但钙黄绿素和3 '-（对氨基苯基）荧光素（APF）的背景信号减少，对CyQUANT和EthD-1荧光响应无影响。磁铁矿使DCFH的荧光信号降低，但在活性氧诱导剂叔丁基过氧化氢（TBHP）存在下，它并不降低荧光信号。相比之下，磁赤铁矿会导致荧光增加，而在抗氧化剂N-乙酰半胱氨酸的存在下，荧光大大减少。本研究强调了考虑和控制NM与荧光/比色染料之间可能的相互作用的重要性，最重要的是，dUSPION的氧化态可能会混淆其灵敏度和特异性。
Due to the unique physicochemical properties of nanomaterials (NM) and their unknown reactivity, the possibility of NM altering the optical properties of fluorometric/colorimetric probes that are used to measure their cyto- and genotoxicity may lead to inaccurate readings. This could have potential implications given that NM, such as ultrafine superparamagnetic iron oxide nanoparticles (USPION), are increasingly finding their use in nanomedicine and the absorbance/fluorescence based assays are used to assess their toxicity. This study looks at the potential of dextran-coated USPION (dUSPION) (maghemite and magnetite) to alter the background signal of common probes used for evaluating cytotoxicity (MTS, CyQUANT, Calcein, and EthD-1) and oxidative stress (DCFH-DA and APF). In the present study, both forms of dUSPION caused an increase in MTS signal but a decrease in background signal from calcein and 3'-(p-aminophenyl) fluorescein (APF) and no effect on CyQUANT and EthD-1 fluorescence responses. Magnetite caused a decrease in fluorescence signal of DCFH, but it did not decrease fluorescence signal in the presence of the reactive oxygen species-inducer tert-butyl hydroperoxide (TBHP). In contrast, maghemite caused an increase in fluorescence, which was substantially reduced in the presence of the antioxidant N-acetyl cysteine. This study emphasizes the importance of considering and controlling for possible interactions between NM and fluorometric/colorimetric dyes and, most importantly, the oxidation state of dUSPION that may confound their sensitivity and specificity.
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影响因子：
13.3
作者：
Hacliipanayis, Coslas G.;Bonder, Michael J.;Balakrishanan, Srinivasan;Wang, Xiaoxia;Mao, Huio;Hadjipanayis, George C.
通讯作者：
Hadjipanayis, George C.
影响因子：
19.7
作者：
Arbab, AS;Bashaw, LA;Frank, JA
通讯作者：
Frank, JA
影响因子：
10.8
作者：
Manna, SK;Sarkar, S;Ramesh, GT
通讯作者：
Ramesh, GT
影响因子：
6.1
作者：
Cassee, FR;Muijser, H;Arts, JHE
通讯作者：
Arts, JHE
影响因子：
3.5
作者：
Karlsson, Hanna L.;Gustafsson, Johanna;Moller, Lennart
通讯作者：
Moller, Lennart