Estimation of shear stress heterogeneity along capillary segments in angiogenic rat mesenteric microvascular networks.
Estimation of shear stress heterogeneity along capillary segments in angiogenic rat mesenteric microvascular networks.
复制标题
估计血管生成大鼠肠系膜微血管网络中沿毛细血管段的剪切应力异质性。
DOI：
10.1111/micc.12830
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Balogh,Peter
中科院分区：
文献类型：
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作者：
Hu,Nien-Wen;Lomel,BanksM;Rice,ElijahW;Hossain,MirMdNasim;Sarntinoranont,Malisa;Secomb,TimothyW;Murfee,WalterL;Balogh,Peter
流体剪切应力被认为是血管生成过程中内皮细胞行为的调节剂。然而，这种联系需要了解血管生成微血管网络中毛细血管水平的应力值。关键问题依然存在。压力是什么？毛细血管是否经历类似的应力大小？变异能解释血管特异性行为吗？本研究的目的是估计在血管生成networks.MethodsImages血管生成网络的特点是增加血管密度的片段特异性剪切应力从大鼠肠系膜组织刺激化合物48/80诱导肥大细胞脱粒。在收获和免疫标记PECAM之前，通过灌注40 kDa可固定的葡聚糖鉴定血管。使用一个网络流为基础的段模型与生理相关的参数，应力计算每个血管跨多个networks.ResultsStresses的区域范围从0.003到2328.1达因/厘米2和变化显着的毛细血管水平。对于所有的区域，最大的节段性剪切应力为毛细管段。应力沿着近端毛细血管分支从小动脉入口增加相比，应力沿着毛细血管在更远的regions.ConclusionsThe结果突出了变化的剪切应力沿着血管生成的毛细血管和激发新的讨论如何内皮细胞可能会在体内响应段特定的微环境血管生成过程中。
ObjectiveFluid shear stress is thought to be a regulator of endothelial cell behavior during angiogenesis. The link, however, requires an understanding of stress values at the capillary level in angiogenic microvascular networks. Critical questions remain. What are the stresses? Do capillaries experience similar stress magnitudes? Can variations explain vessel‐specific behavior? The objective of this study was to estimate segment‐specific shear stresses in angiogenic networks.MethodsImages of angiogenic networks characterized by increased vascular density were obtained from rat mesenteric tissues stimulated by compound 48/80‐induced mast cell degranulation. Vessels were identified by perfusion of a 40 kDa fixable dextran prior to harvesting and immunolabeling for PECAM. Using a network flow‐based segment model with physiologically relevant parameters, stresses were computed per vessel for regions across multiple networks.ResultsStresses ranged from 0.003 to 2328.1 dyne/cm2and varied dramatically at the capillary level. For all regions, the maximum segmental shear stresses were for capillary segments. Stresses along proximal capillaries branching from arteriole inlets were increased compared to stresses along capillaries in more distal regions.ConclusionsThe results highlight the variability of shear stresses along angiogenic capillaries and motivate new discussions on how endothelial cells may respond in vivo to segment‐specific microenvironment during angiogenesis.