Localization of tartrate-resistant acid phosphatase in human placenta
Localization of tartrate-resistant acid phosphatase in human placenta
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DOI：
10.1007/bf02331443
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发表时间：
1996-03-01
期刊：
影响因子：
--
通讯作者：
Yam, LT
中科院分区：
文献类型：
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作者：
Janckila, AJ;Yaziji, H;Yam, LT
抗酒石酸酸性磷酸酶是巨噬细胞系特化细胞和部分活化淋巴细胞表达的细胞分化和活化的诱导标志物。临床上，这种磷酸酶是毛细胞白血病和破骨细胞活性的诊断标志物。该酶的cDNA已从胎盘表达文库中克隆出来，但表达该酶蛋白的细胞尚未确定。我们的实验室开发了一种单克隆抗体9C5，适用于石蜡切片中抗酒石酸酸性磷酸酶的免疫组织化学定位。本研究的目的是利用抗体9C5在石蜡包埋的正常足月胎盘切片中鉴定表达抗酒石酸酸性磷酸酶的细胞，并确定这些细胞是否表达其他巨噬细胞标志物，包括CD68 （PG-M1抗体）、LN5、溶菌酶、α(1)-抗胰蛋白酶和α(1)-抗凝乳胰蛋白酶。将冷冻切片的组织化学定位与同一组织标本石蜡切片的免疫组织化学定位进行比较。该酶的活性和抗原性在蜕细胞、合体滋养细胞和分布在母体和胚胎组织中的一些巨噬细胞中检测到，但在中性粒细胞中未检测到。与先前检查的其他组织不同，胎盘含有大量的非巨噬细胞来源的磷酸盐阳性细胞。
Tartrate-resistant acid phosphatase is an inducible marker of cell differentiation and activation expressed by specialized cells of macrophage Lineage and some activated lymphocytes. Clinically, this phosphatase is a diagnostic marker for hairy cell leukaemia and osteoclast activity. The cDNA for this enzyme has been cloned from a placental expression library, yet the cell(s) expressing the enzyme protein has not been determined with certainty. Our laboratories have developed a monoclonal antibody, 9C5, suitable for immunohistochemical localization of tartrate-resistant acid phosphatase in paraffin sections. The purpose of this study was to use antibody 9C5 to identify cells expressing tartrate-resistant acid phosphatase in sections of paraffin-embedded, normal, full-term placenta and to determine if those cells expressed other macrophage markers including CD68 (PG-M1 antibody), LN5, lysozyme, alpha(1)-antitrypsin and alpha(1)-antichymotrypsin. Histochemical localization of activity in frozen sections was compared with immunohistochemical localization in paraffin sections of the same tissue specimens. The activity and antigenicity of this enzyme were detected in decidual cells, syncytiotrophoblast, and some macrophages distributed throughout maternal and embryonic tissues, but not in neutrophils. Unlike other tissues previously examined, placenta contains significant numbers of the phosphate-positive cells that are not of macrophage origin.