Production and characterization of a low-molecular-weight bacteriocin from Bacillus licheniformis MKU3
Production and characterization of a low-molecular-weight bacteriocin from Bacillus licheniformis MKU3
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DOI：
10.1111/j.1472-765x.2008.02473.x
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发表时间：
2008-12-01
影响因子：
2.4
通讯作者：
Gunasekaran, P.
中科院分区：
文献类型：
--
作者：
Kayalvizhi, N.;Gunasekaran, P.
提高地衣芽孢杆菌MKU3中低分子细菌素的产量和性质。地衣芽孢杆菌MKU3的培养上清液对革兰氏阳性菌和阴性菌以及不同的真菌和酵母菌具有类细菌素活性。地衣芽孢杆菌MKU3胞外蛋白的SDS-PAGE分析表明，MKU3胞外蛋白为细菌素样蛋白，相对分子质量为1.5 kDa。地衣芽孢杆菌MKU3的细菌素活性在pH为3.0-10.0的范围内保持稳定，在100℃的温度下保持60min，但被蛋白酶K、胰酶或链霉蛋白酶E灭活。地衣芽孢杆菌MKU3的细菌素活性最高(11000 AU ml(-1))。地衣芽孢杆菌MKU3的低相对分子质量类细菌素蛋白对几种革兰氏阳性细菌、几种真菌和酵母具有广泛的抗菌活性。通过部分析因设计优化，细菌素的产量提高了3.6倍。对革兰氏阳性细菌、丝状真菌和酵母菌具有广谱活性的细菌素具有潜在的临床应用前景。统计方法有助于优化细菌素生产的培养基数。
Enhancing production and characterization of a low-molecular-weight bacteriocin from Bacillus licheniformis MKU3.The culture supernatant of B. licheniformis MKU3 exhibited bacteriocin-like activity against Gram-positive and -negative bacteria and different fungi and yeast. SDS-PAGE analysis of the extracellular proteins of B. licheniformis MKU3 revealed a bacteriocin-like protein with a molecular mass of 1.5 kDa. This bacteriocin activity was found to be stable under a pH range of 3.0-10.0 and at temperatures up to 100 degrees C for 60 min, but inactivated by proteinase K, trypsin or pronase E. An experimental fractional factorial design for optimization of production medium resulted in a maximum activity of bacteriocin (11 000 AU ml(-1)) by B. licheniformis MKU3.A low-molecular-weight bacteriocin-like protein from B. licheniformis MKU3 exhibited a wide spectrum of antimicrobial activity against several Gram-positive bacteria, several fungi and yeast. A 3.6-fold increase in the production of bacteriocin was achieved using the culture medium optimized through a fractional factorial design.A bacteriocin with wide spectrum of activity against Gram-positive bacterial pathogens, filamentous fungi and yeast suggested its potential clinical use. Statistical method facilitated optimization of cultural medium for the improved production of bacteriocin.