Drosophila Dosage Compensation Involves Enhanced Pol II Recruitment to Male X-Linked Promoters
Drosophila Dosage Compensation Involves Enhanced Pol II Recruitment to Male X-Linked Promoters
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DOI：
10.1126/science.1221428
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发表时间：
2012-08-10
期刊：
影响因子：
56.9
通讯作者：
Akhtar, Asifa
中科院分区：
文献类型：
--
作者：
Conrad, Thomas;Cavalli, Florence M. G.;Akhtar, Asifa
通过组蛋白H4赖氨酸16（H4 K16）的超乙酰化，果蝇中的雄性特异性致死（MSL）复合物使来自单个雄性X染色体的转录大约加倍，以匹配雌性中的X连锁表达和来自二倍体常染色体的表达。通过获得准确的测量RNA聚合酶II（Pol II）occupancy和短启动子近端RNA生产，我们检测到一个一致的，基因组规模的增加Pol II活性在男性X连锁基因的启动子。此外，我们发现，增强Pol II招聘男性X-连锁启动子在很大程度上依赖于MSL复合物。这些观察提供了深入了解如何通过组蛋白乙酰化对染色质结构进行全局调节，从而有助于精确控制Pol II功能。
Through hyperacetylation of histone H4 lysine 16 (H4K16), the male-specific lethal (MSL) complex in Drosophila approximately doubles transcription from the single male X chromosome in order to match X-linked expression in females and expression from diploid autosomes. By obtaining accurate measurements of RNA polymerase II (Pol II) occupancies and short promoter-proximal RNA production, we detected a consistent, genome-scale increase in Pol II activity at the promoters of male X-linked genes. Moreover, we found that enhanced Pol II recruitment to male X-linked promoters is largely dependent on the MSL complex. These observations provide insights into how global modulation of chromatin structure by histone acetylation contributes to the precise control of Pol II function.