Neuropeptide F Neurons Modulate Sugar Reward During Associative Olfactory Learning of Drosophila Larvae
Neuropeptide F Neurons Modulate Sugar Reward During Associative Olfactory Learning of Drosophila Larvae
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DOI：
10.1002/cne.23873
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发表时间：
2015-12-15
影响因子：
2.5
通讯作者：
Thum, Andreas S.
中科院分区：
文献类型：
--
作者：
Rohwedder, Astrid;Selcho, Mareike;Thum, Andreas S.
为了生存，所有的有机体都必须不断地根据环境的变化来调整它们的行为。经验驱动的行为变化通常是由大脑回路中信号的改变来调节和维持的。鉴于果蝇幼虫大脑的简单性及其在遗传和行为水平上的实验可及性，我们分析了果蝇神经肽F(DNPF)神经元是否参与了经典的嗅觉条件反射。DNPF是哺乳动物神经肽Y的同源基因，神经肽Y是一种高度保守的神经调节剂，刺激觅食行为。我们在单细胞水平上对dNPF神经元进行了全面的解剖分析。我们证明，人工激活dNPF神经元通过调节获取过程中的糖奖励信号来抑制食欲嗅觉学习。没有检测到恢复已建立的食欲嗅觉记忆的效果。这种调节作用是基于三种不同细胞类型的联合作用，如果在单细胞水平上进行测试，它们会抑制和逆转条件性行为。综上所述，我们的工作从解剖和功能上描述了果蝇幼虫经典嗅觉调节中糖强化信号通路的一个新部分。(C)2015年威利期刊公司。
All organisms continuously have to adapt their behavior according to changes in the environment in order to survive. Experience-driven changes in behavior are usually mediated and maintained by modifications in signaling within defined brain circuits. Given the simplicity of the larval brain of Drosophila and its experimental accessibility on the genetic and behavioral level, we analyzed if Drosophila neuropeptide F (dNPF) neurons are involved in classical olfactory conditioning. dNPF is an ortholog of the mammalian neuropeptide Y, a highly conserved neuromodulator that stimulates food-seeking behavior. We provide a comprehensive anatomical analysis of the dNPF neurons on the single-cell level. We demonstrate that artificial activation of dNPF neurons inhibits appetitive olfactory learning by modulating the sugar reward signal during acquisition. No effect is detectable for the retrieval of an established appetitive olfactory memory. The modulatory effect is based on the joint action of three distinct cell types that, if tested on the single-cell level, inhibit and invert the conditioned behavior. Taken together, our work describes anatomically and functionally a new part of the sugar reinforcement signaling pathway for classical olfactory conditioning in Drosophila larvae. (C) 2015 Wiley Periodicals, Inc.