Expression and Characterization of Mammalian Carotenoid Cleavage Dioxygenases.
Expression and Characterization of Mammalian Carotenoid Cleavage Dioxygenases.
复制标题
哺乳动物类胡萝卜素裂解双加氧酶的表达和表征。
DOI：
10.1007/978-1-4939-9952-1_6
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
vonLintig,Johannes
中科院分区：
文献类型：
--
作者：
Thomas,LindaDora;Ramkumar,Srinivasagan;vonLintig,Johannes
类胡萝卜素裂解双加氧酶(CCDs)是催化类胡萝卜素及其类胡萝卜素代谢产物双键加工的非血红素铁酶。哺乳动物基因组编码该蛋白家族的三个成员，即BCO1、BCO2和RPE65。相应基因的突变和遗传多态与遗传性致盲疾病、维生素A缺乏和高胡萝卜素血浆水平有关。在这里，我们描述了一种哺乳动物BCO1和BCO2 ine的异源表达方法。并对这些重组酶的生化特性进行了研究。解剖CCDs的酶特性将促进我们对由这些疾病相关酶控制的其他生化过程的了解，并可能有助于针对这些疾病状态的干预措施的设计。
Carotenoid cleavage dioxygenases (CCDs) are nonheme iron enzymes that catalyze double bond processing of carotenoids and their apocarotenoid metabolites. Mammalian genomes encode three members of this protein family, namely BCO1, BCO2, and RPE65. Mutations and genetic polymorphism in the corresponding genes are associated with inherited blinding diseases, vitamin A deficiency, and high carotenoid plasma levels. Here we describe a method for the heterologous expression of mammalian BCO1 and BCO2 inE. coliand the biochemical characterization of these recombinant enzymes. Dissecting the enzymatic properties of CCDs will advance our knowledge of the biochemical processes that are govern by these disease-associated enzymes and may assist the design of interventions directed against these disease states.