VACCINIA VIRUS GENE-B1R ENCODES A 34-KDA SERINE THREONINE PROTEIN-KINASE THAT LOCALIZES IN CYTOPLASMIC FACTORIES AND IS PACKAGED INTO VIRIONS
VACCINIA VIRUS GENE-B1R ENCODES A 34-KDA SERINE THREONINE PROTEIN-KINASE THAT LOCALIZES IN CYTOPLASMIC FACTORIES AND IS PACKAGED INTO VIRIONS
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DOI：
10.1016/0042-6822(92)90256-o
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发表时间：
1992-12-01
期刊：
影响因子：
3.7
通讯作者：
SMITH, GL
中科院分区：
文献类型：
--
作者：
BANHAM, AH;SMITH, GL
痘苗病毒开放阅读框B1R在大肠杆菌中表达。大肠杆菌编码丝氨酸/苏氨酸蛋白激酶，可在体外磷酸化酪蛋白和小牛胸腺组蛋白。针对 TrpE-B1R 细菌融合蛋白产生多克隆兔抗血清，并用于表征 B1R 基因产物。免疫沉淀和免疫印迹分析检测到在痘苗病毒感染早期合成的 34-kDa 多肽，并且由于在感染后 18 小时很容易检测到，因此该多肽显然是稳定的。免疫荧光表明该蛋白定位于细胞质病毒工厂，即病毒 DNA 复制的场所。痘苗病毒粒子的免疫印迹显示该酶被包装成病毒颗粒。
Vaccinia virus open reading frame B1R was expressed inE. coliand shown to encode a serine/threonine protein kinase which phosphorylated casein and calf thymus histonesin vitro. A polyclonal rabbit antiserum was raised against a TrpE-B1R bacterial fusion protein and used to characterize the B1R gene product. Immunoprecipitation and immunoblotting analyses detected a 34-kDa polypeptide that was synthesized early during vaccinia virus infection and which was apparently stable since it was easily detectable 18 hr postinfection. Immunofluorescence demonstrated that this protein localizes in cytoplasmic virus factories, the sites of virus DNA replication. Immunoblotting of vaccinia virions showed that the enzyme is packaged into virus particles.