Dicer is dispensable for asymmetric RISC loading in mammals
Dicer is dispensable for asymmetric RISC loading in mammals
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DOI：
10.1261/rna.029785.111
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发表时间：
2012-01-01
期刊：
影响因子：
4.5
通讯作者：
Tomari, Yukihide
中科院分区：
文献类型：
--
作者：
Betancur, Juan G.;Tomari, Yukihide
在果蝇中，将小RNA双链体不对称加载到含有Argonaute 2的RNA诱导沉默复合物（Ago 2-RISC）中需要Dicer-2/R2D2异二聚体，其充当小RNA双链体末端的热力学稳定性的蛋白质传感器。然而，哺乳动物RISC组装中小RNA不对称感测的机制仍然不清楚。在这里，我们定量检测了哺乳动物中存在或不存在Dicer的RISC组装和靶沉默活性。我们的数据显示，与充分表征的果蝇Ago 2-RISC组装途径不同，哺乳动物Dicer在体内和体外不对称RISC加载。
In flies, asymmetric loading of small RNA duplexes into Argonaute2-containing RNA-induced silencing complex (Ago2-RISC) requires Dicer-2/R2D2 heterodimer, which acts as a protein sensor for the thermodynamic stabilities of the ends of small RNA duplexes. However, the mechanism of small RNA asymmetry sensing in mammalian RISC assembly remains obscure. Here, we quantitatively examined RISC assembly and target silencing activity in the presence or absence of Dicer in mammals. Our data show that, unlike the well-characterized fly Ago2-RISC assembly pathway, mammalian Dicer is dispensable for asymmetric RISC loading in vivo and in vitro.