Modulation of Rat Cecal Microbiota by Administration of Raffinose and Encapsulated Bifidobacterium breve
Modulation of Rat Cecal Microbiota by Administration of Raffinose and Encapsulated Bifidobacterium breve
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DOI：
10.1128/aem.72.1.784-792.2006
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发表时间：
2006-01
影响因子：
4.4
通讯作者：
A. Dinoto;A. Suksomcheep;S. Ishizuka;H. Kimura;S. Hanada;Y. Kamagata;Kozo Asano;F. Tomita;A. Yokota
中科院分区：
文献类型：
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作者：
A. Dinoto;A. Suksomcheep;S. Ishizuka;H. Kimura;S. Hanada;Y. Kamagata;Kozo Asano;F. Tomita;A. Yokota
摘要为了研究棉籽糖和胶囊化短双歧杆菌JCM 1192 T细胞对大鼠盲肠微生物群的影响，在临床前合生素研究中，雄性WKAH/Hkm Slc大鼠组用四种不同的测试饮食喂养3周：基础饮食（BD组）、补充有棉籽糖的基础饮食（RAF组）、补充有胶囊化B的基础饮食。CB组，基础日粮中同时添加棉籽糖和微胶囊B。RCB组（breve）。采用荧光原位杂交（FISH）和末端限制性片段长度多态性（T-RFLP）技术检测盲肠标本中的细菌种群。B。FISH分析显示，RCB组中有短细胞，约占总细胞的6.3%。B。T-RFLP分析也仅在RCB组中检测到breve。这与CB组相反，在CB组中没有B。FISH或T-RFLP检测到短信号。在RAF和RCB组中观察到动物双歧杆菌（一种大鼠特有的双歧杆菌）种群数量增加。T-RFLP结果的主成分分析显示RAF和RCB组大鼠的细菌种群发生了显著变化; CB组的种群与对照组（BD组）相似。据我们所知，这些结果提供了第一个明确的图片，在大鼠盲肠微生物群的变化，以响应合生素管理。
ABSTRACT To investigate the effects of administration of raffinose and encapsulated Bifidobacterium breve JCM 1192T cells on the rat cecal microbiota, in a preclinical synbiotic study groups of male WKAH/Hkm Slc rats were fed for 3 weeks with four different test diets: basal diet (group BD), basal diet supplemented with raffinose (group RAF), basal diet supplemented with encapsulated B. breve (group CB), and basal diet supplemented with both raffinose and encapsulated B. breve (group RCB). The bacterial populations in cecal samples were determined by fluorescence in situ hybridization (FISH) and terminal restriction fragment length polymorphism (T-RFLP). B. breve cells were detected only in the RCB group and accounted for about 6.3% of the total cells as determined by FISH analysis. B. breve was also detected only in the RCB group by T-RFLP analysis. This was in contrast to the CB group, in which no B. breve signals were detected by either FISH or T-RFLP. Increases in the sizes of the populations of Bifidobacterium animalis, a Bifidobacterium indigenous to the rat, were observed in the RAF and RCB groups. Principal-component analysis of T-RFLP results revealed significant alterations in the bacterial populations of rats in the RAF and RCB groups; the population in the CB group was similar to that in the control group (group BD). To the best of our knowledge, these results provide the first clear picture of the changes in the rat cecal microbiota in response to synbiotic administration.