Bactericidal Action of Photodynamic Antimicrobial Chemotherapy (PACT) with Photosensitizers Used as Plaque-Disclosing Agents against Experimental Biofilm
Bactericidal Action of Photodynamic Antimicrobial Chemotherapy (PACT) with Photosensitizers Used as Plaque-Disclosing Agents against Experimental Biofilm
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DOI：
10.4265/bio.21.187
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发表时间：
2016-09-01
影响因子：
1.2
通讯作者：
Niwano, Yoshimi
中科院分区：
文献类型：
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作者：
Ishiyama, Kirika;Nakamura, Keisuke;Niwano, Yoshimi
本研究发现，作为牙菌斑暴露剂的玫瑰红、红、菲辛、杂蒽光敏剂在光照射下可通过单线态氧对浮游变形链球菌产生杀菌作用。在酸性电解水（AcEW）和碱性电解水（AIEW）的联合作用下，研究了三种杂蒽类化合物对变形链球菌实验生物膜的杀菌活性。结果表明，在AIEW中，只有光照射的红玫瑰表现出显著的杀菌活性，活菌数量减少了100 - 3倍。由于我们之前的研究表明，玫瑰花对细菌细胞的亲和力优于红素和菲奥辛，推测AIEW破坏了实验生物膜的细胞外基质，使玫瑰花容易与细菌细胞结合。从这些结果，强烈建议玫瑰是一个合适的光敏剂作为菌斑暴露剂在光动力抗菌化疗治疗牙菌斑。
Our previous study revealed that the photo-irradiation of rose bengal, erythrosine, and phioxine, xanthene photosensitizers, used as dental plaque disclosing agents, could exert bactericidal action on planktonic Streptococcus mutans via the singlet oxygen. In the present study, the photo-irradiation induced bactericidal activity of the three xanthene compounds against the experimental biofilm of S. mutans was investigated in combination with acid electrolyzed water (AcEW) and alkaline electrolyzed water (AIEW). As a result, only the photo-irradiated rose bengal in AIEW showed prominent bactericidal activity with a >3-log reduction of the viable bacterial count. Since our previous study showed that the affinity of rose bengal to bacterial cells was superior to that of erythrosine and phioxine, it was speculated that AIEW damaged the extracellular matrix of the experimental biofilm, which would let the rose bengal easily be bound to the bacterial cells. From these results, it is strongly suggested that rose bengal is a suitable photosensitizer for use as a plaque disclosing agent in photodynamic antimicrobial chemotherapy to treat dental plaque.