Bone Regeneration in rat using a gelatin/bioactive glass nanocomposite scaffold along with endothelial cells (HUVECs)
Bone Regeneration in rat using a gelatin/bioactive glass nanocomposite scaffold along with endothelial cells (HUVECs)
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DOI：
10.1111/ijac.12907
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发表时间：
2018-11-01
影响因子：
2.1
通讯作者：
Farajollahi, Mohammad
中科院分区：
文献类型：
--
作者：
Kazemi, Mansure;Azami, Mahmoud;Farajollahi, Mohammad
在我们前期的研究中，通过层状溶剂浇注结合层压技术制备了总孔隙率约为85%、孔径在200~500m之间的三维明胶/生物活性玻璃纳米复合支架。本研究的目的是评价含内皮细胞和不含内皮细胞的支架植入临界大鼠颅骨缺损后的体外生物相容性和体内骨再生能力。通过四甲基偶氮唑盐比色法、扫描电子显微镜观察和DAPI染色检测细胞在大孔支架中的存活和黏附情况，结果表明该支架具有足够的生物相容性，能够支持细胞的贴壁和增殖。为了研究支架的体内成骨作用，将空白支架和内皮/支架结构植入临界大小的缺损区，而对照组的缺损区不进行处理。通过组织学、免疫组织化学和组织形态计量学分析，在术后1、4和12周评价骨再生和血管形成。结果表明，两组都促进了骨生长进入缺损区，但内皮细胞的加入促进了骨再生。结果表明，多孔Gel/Bag纳米复合支架能够很好地支持新骨形成，是组织工程化骨缺损的一种有前景的替代方案。
In our previous study, a three-dimensional gelatin/bioactive glass nanocomposite scaffold with a total porosity of about 85% and pore sizes ranging from 200 to 500m was prepared through layer solvent casting combined with lamination technique. The aim of this study was to evaluate invitro biocompatibility and invivo bone regeneration potential of these scaffolds with and without endothelial cells when implanted into a critical-sized rat calvarial defect. MTT assay, SEM observation, and DAPI staining were used to evaluate cell viability and adhesion in macroporous scaffolds and results demonstrated that the scaffolds were biocompatible enough to support cell attachment and proliferation. To investigate the invivo osteogenesis of the scaffold, blank scaffolds and endothelial/scaffold constructs were implanted in critical-sized defects, whereas in control group defects were left untreated. Bone regeneration and vascularization were evaluated at 1, 4, and 12weeks postsurgery by histological, immunohistochemical, and histomorphometric analysis. It was shown that both groups facilitated bone growth into the defect area but improved bone regeneration was seen with the incorporation of endothelial cells. The data showed that the porous Gel/BaG nanocomposite scaffolds could well support new bone formation, indicating that the proposed strategy is a promising alternative for tissue-engineered bone defects.