Pretreatment with adenosine and adenosine A1 receptor agonist protects against intestinal ischemia-reperfusion injury in rat
Pretreatment with adenosine and adenosine A1 receptor agonist protects against intestinal ischemia-reperfusion injury in rat
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DOI：
10.3748/wjg.v13.i4.538
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发表时间：
2007-01-28
影响因子：
4.3
通讯作者：
Sayan, Hale
中科院分区：
文献类型：
--
作者：
Ozacmak, V. Haktan;Sayan, Hale
目的：方法：大鼠随机分为假手术组、缺血再灌注组和缺血前分别给予腺苷、A1受体激动剂2-氯-N-6-环戊基腺苷、A1受体拮抗剂8-环戊基-1，3-二丙基黄嘌呤和腺苷治疗组，观察腺苷和A1受体激活对再灌注小肠损伤的影响。再灌注后，记录回肠对KCl、卡巴胆碱和P物质的反应。组织髓过氧化物酶，丙二醛，还原型谷胱甘肽水平进行了measured.Results：缺血显着降低收缩和还原型谷胱甘肽水平，这是改善腺苷和激动剂管理。治疗还减少了中性粒细胞浸润和膜脂质过氧化。A1受体拮抗剂预处理可消除腺苷的有益作用。结论：腺苷和A1受体刺激可通过降低氧化应激、减少中性粒细胞浸润和增加还原型谷胱甘肽含量减轻缺血性肠损伤。(c)2007年，WJG出版社。All rights reserved.
AIM: To examine the effects of adenosine and A1 receptor activation on reperfusion-induced small intestinal injury.METHODS: Rats were randomized into groups with sham operation, ischemia and reperfusion, and systemic treatments with either adenosine or 2-chloro-N-6-cyclo- pentyladenosine, A1 receptor agonist or 8-cyclopentyl-1,3-dipropylxanthine, A1 receptor antagonist, plus adenosine before ischemia. Following reperfusion, contractions of ileum segments in response to KCl, carbachol and substance P were recorded. Tissue myeloperoxidase, malondialdehyde, and reduced glutathione levels were measured.RESULTS: Ischemia significantly decreased both contraction and reduced glutathione level which were ameliorated by adenosine and agonist administration. Treatment also decreased neutrophil infiltration and membrane lipid peroxidation. Beneficial effects of adenosine were abolished by pretreatment with A1 receptor antagonist.CONCLUSION: The data suggest that adenosine and A1 receptor stimulation attenuate ischemic intestinal injury via decreasing oxidative stress, lowering neutrophil infiltration, and increasing reduced glutathione content. (c) 2007 The WJG Press. All rights reserved.