The phenazine pyocyanin is a terminal signalling factor in the quorum sensing network of Pseudomonas aeruginosa
The phenazine pyocyanin is a terminal signalling factor in the quorum sensing network of Pseudomonas aeruginosa
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DOI：
10.1111/j.1365-2958.2006.05306.x
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发表时间：
2006-09-01
影响因子：
3.6
通讯作者：
Newman, Dianne K.
中科院分区：
文献类型：
--
作者：
Dietrich, Lars E. P.;Price-Whelan, Alexa;Newman, Dianne K.
荧光假单胞菌的某些成员产生并分泌吩嗪。这些杂环的氧化还原活性化合物对竞争生物体是有毒的，这些抗生素作用的原因一直是密集研究工作的焦点。然而，假单胞菌本身如何对这些化合物作出反应并在存在这些化合物的情况下生存，这在很大程度上是未知的。使用铜绿假单胞菌DNA微阵列和定量RT-PCR，我们证明，吩嗪绿脓菌素elefines上调基因/操纵子的功能，在运输[如耐药-增殖-细胞分裂（RND）外排泵MexGHI-OpmD]，并可能在氧化还原控制（如PA 2274，一个假定的黄素依赖性单加氧酶），并下调基因参与三价铁的收购。引人注目的是，mexGHI-opmD和PA 2274先前被证明是由控制毒力因子（包括吩嗪）产生的PA 14群体感应网络调节的。通过突变分析，我们表明，绿脓菌素是这些群体感应控制的基因在稳定期上调的生理信号，并介导的转录因子SoxR的反应。我们的研究结果暗示吩嗪作为信号分子在铜绿假单胞菌PA 14和PAO 1。
Certain members of the fluorescent pseudomonads produce and secrete phenazines. These heterocyclic, redox-active compounds are toxic to competing organisms, and the cause of these antibiotic effects has been the focus of intense research efforts. It is largely unknown, however, how pseudomonads themselves respond to - and survive in the presence of - these compounds. Using Pseudomonas aeruginosa DNA microarrays and quantitative RT-PCR, we demonstrate that the phenazine pyocyanin elicits the upregulation of genes/operons that function in transport [such as the resistance-nodulation-cell division (RND) efflux pump MexGHI-OpmD] and possibly in redox control (such as PA2274, a putative flavin-dependant monooxygenase), and downregulates genes involved in ferric iron acquisition. Strikingly, mexGHI-opmD and PA2274 were previously shown to be regulated by the PA14 quorum sensing network that controls the production of virulence factors (including phenazines). Through mutational analysis, we show that pyocyanin is the physiological signal for the upregulation of these quorum sensing-controlled genes during stationary phase and that the response is mediated by the transcription factor SoxR. Our results implicate phenazines as signalling molecules in both P. aeruginosa PA14 and PAO1.