Selective, user-friendly, highly porous, efficient, and rapid (SUPER) filter for isolation and analysis of rare tumor cells
Selective, user-friendly, highly porous, efficient, and rapid (SUPER) filter for isolation and analysis of rare tumor cells
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用于分离和分析稀有肿瘤细胞的选择性、用户友好型、高孔隙度、高效、快速 (SUPER) 过滤器
DOI：
10.1039/d1lc00886b
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发表时间：
2022
期刊：
影响因子：
6.1
通讯作者：
Yang Chaoyong
中科院分区：
文献类型：
--
作者：
Zhao Kaifeng;Liu Yaoping;Wang Hua;Song Yanling;Chen Xiaofeng;Huang Chen;Niu Qi;Cao Jiao;Chen Xin;Wang Wei;Wu Lingling;Yang Chaoyong
基于肿瘤细胞的液体活检技术的临床应用迫切需要从复杂体液中快速、高效、选择性地分离肿瘤细胞。为此，开发了一种用于高性能肿瘤细胞分离和分析的大小选择性亲和过滤系统(Super Filter)，该系统名为选择性、用户友好、高孔、高效和快速的过滤器(Super Filter)。在过滤过程中，超滤使肿瘤细胞与尺寸优化和抗体包被的微孔壁选择性地相互作用，在缓冲液中的效率为91.0±6.1%，在全血中的效率为83.7±6.4%。同时，与传统过滤装置相比，其更大的微孔尺寸大大降低了对背景细胞的非特异性捕获(每毫升血液中有55-126个细胞)，浓缩倍数为1.1×104-1.0×105，纯度为52.7±4.2%。此外，其高孔隙率实现了超快速(1毫升血液或10毫升缓冲样本的5 S)和用户友好的重力驱动过滤。最后，Super Filter展示了快速、高效和选择性地从血液中分离肿瘤细胞以及从癌症患者的大量胸腹水样本中进行形态和分子分析。我们期望这种大小选择性亲和过滤策略能够促进基于肿瘤细胞的液体活检的临床应用。
Rapid, efficient, and selective separation of tumor cells from complex body fluids is urgently needed for clinical application of tumor-cell-based liquid biopsy. Herein, a size-selective affinity filtration system, named selective, user-friendly, highly porous, efficient, and rapid filter (SUPER Filter), was developed for high-performance tumor cell isolation and analysis. SUPER Filter enabled selective interaction of tumor cells with size-optimized and antibody-coated micropore walls during filtration, achieving a high efficiency of 91.0 ± 6.1% in buffer and 83.7 ± 6.4% in whole blood. Meanwhile, its larger micropore size than those of traditional filtration devices greatly reduced the nonspecific capture of background cells (55–126 cells per mL blood) with enrichment factors of 1.1 × 104–1.0 × 105 and a purity of 52.7 ± 4.2%. Moreover, its high porosity enabled ultra-fast (<5 s for 1 mL of blood or 10 mL of buffer samples) and user-friendly gravity-driven filtration. Finally, SUPER Filter demonstrated rapid, efficient, and selective separation of tumor cells from blood and large-volume pleural and ascetic fluid samples from cancer patients for morphological and molecular analysis. We expect that this size-selective affinity filtration strategy facilitates the clinical application of tumor-cell-based liquid biopsy.