MAST-CELLS AS A SOURCE OF BOTH PREFORMED AND IMMUNOLOGICALLY INDUCIBLE TNF-ALPHA CACHECTIN
MAST-CELLS AS A SOURCE OF BOTH PREFORMED AND IMMUNOLOGICALLY INDUCIBLE TNF-ALPHA CACHECTIN
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DOI：
10.1038/346274a0
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发表时间：
1990-07-19
期刊：
影响因子：
64.8
通讯作者：
GALLI, SJ
中科院分区：
文献类型：
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作者：
GORDON, JR;GALLI, SJ
肿瘤坏死因子-α（TNF-α）/恶病质素是一种多功能细胞因子，在炎症、脓毒症、脂质和蛋白质代谢、造血、血管生成和宿主对寄生虫和恶性肿瘤的抵抗力中发挥作用1 -3。TNF-α首先在活化的巨噬细胞中被描述1 -3，但某些小鼠或大鼠肥大细胞群（综述见参考文献4、5）和一些具有肥大细胞-嗜碱性细胞化学特征的体外衍生人类细胞6也可能含有类似于TNF-α的产物。在这里，我们提出的证据表明，居民小鼠腹腔肥大细胞组成性含有大量的TNF-α的生物活性，而培养的，未成熟的肥大细胞在其TNF-α含量的变化。培养的或腹膜肥大细胞的IgE依赖性活化7诱导TNF-α的细胞外释放，并增加TNF-α信使RNA水平和生物活性。这些发现表明小鼠肥大细胞是预先形成的和免疫诱导的TNF-α的重要来源，并表明肥大细胞释放TNF-α可能有助于宿主防御、过敏性疾病的病理生理学和其他依赖于TNF-α的过程。
TUMOUR necrosis factor-α (TNF-α)/cachectin is a multifunctional cytokine that has effects in inflammation, sepsis, lipid and protein metabolism, haematopoiesis, angiogenesis and host resistance to parasites and malignancy1–3. TNF-α was first described in activated macrophages1–3, but certain mouse or rat mast cell populations (reviewed in refs 4,5) and somein vitro-derived human cells with cytochemical features of mast cells-basophils6may also contain products similar to TNF-α. Here we present evidence that resident mouse peritoneal mast cells constitutively contain large amounts of TNF-α bioactivity, whereas cultured, immature mast cells vary in their TNF-α content. IgE-dependent activation of cultured or peritoneal mast cells7induces extracellular release of TNF-α and augments levels of TNF-α messenger RNA and bioactivity. These findings identify mouse mast cells as an important source of both preformed and immunologically inducible TNF-α, and suggest that release of TNF-α by mast cells may contribute to host defence, the pathophysiology of allergic diseases and other processes dependent on TNF-α.