High-Density Lipoprotein Binds to Mycobacterium avium and Affects the Infection of THP-1 Macrophages.
High-Density Lipoprotein Binds to Mycobacterium avium and Affects the Infection of THP-1 Macrophages.
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DOI：
10.1155/2016/4353620
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发表时间：
2016
影响因子：
5.3
通讯作者：
Tozuka M
中科院分区：
文献类型：
--
作者：
Ichimura N;Sato M;Yoshimoto A;Yano K;Ohkawa R;Kasama T;Tozuka M
高密度脂蛋白（HDL）参与对各种感染性疾病的先天免疫。关于细菌，已知HDL与脂多糖（LPS）结合并中和其生理活性。另一方面，已知胆固醇在分枝杆菌进入宿主细胞和在细胞内环境中存活中起重要作用。然而，鸟分枝杆菌（M.禽流感）感染，这往往是世界范围内增加，仍然缺乏研究。在这里，我们报告说，HDL表明与M更强的相互作用。与其它含丰富LPS的革兰阴性菌相比，载脂蛋白A-I是HDL的主要蛋白成分，与M. Avium可能参与了这种相互作用。HDL对M无直接杀菌活性。avium，但减弱M.通过THP-1巨噬细胞。高密度脂蛋白也没有影响细菌杀死后摄入活M。通过THP-1巨噬细胞的作用。此外，HDL强烈促进M.禽流感感染的THP-1巨噬细胞。这些观察为M.禽流感病毒感染和宿主脂蛋白，特别是HDL。因此，HDL可以帮助M。逃避宿主的先天免疫。
High-density lipoprotein (HDL) is involved in innate immunity toward various infectious diseases. Concerning bacteria, HDL is known to bind to lipopolysaccharide (LPS) and to neutralize its physiological activity. On the other hand, cholesterol is known to play an important role in mycobacterial entry into host cells and in survival in the intracellular environment. However, the pathogenicity of Mycobacterium avium (M. avium) infection, which tends to increase worldwide, remains poorly studied. Here we report that HDL indicated a stronger interaction with M. avium than that with other Gram-negative bacteria containing abundant LPS. A binding of apolipoprotein (apo) A-I, the main protein component of HDL, with a specific lipid of M. avium might participate in this interaction. HDL did not have a direct bactericidal activity toward M. avium but attenuated the engulfment of M. avium by THP-1 macrophages. HDL also did not affect bacterial killing after ingestion of live M. avium by THP-1 macrophage. Furthermore, HDL strongly promoted the formation of lipid droplets in M. avium-infected THP-1 macrophages. These observations provide new insights into the relationship between M. avium infection and host lipoproteins, especially HDL. Thus, HDL may help M. avium to escape from host innate immunity.