Microbial community structure in an integrated A/O reactor treating diluted livestock wastewater during start-up period.
Microbial community structure in an integrated A/O reactor treating diluted livestock wastewater during start-up period.
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DOI：
10.1016/s1001-0742(09)60159-5
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发表时间：
2010
影响因子：
6.9
通讯作者：
Lijiao Ren;Yining Wu;N. Ren;Kun Zhang;D. Xing
中科院分区：
文献类型：
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作者：
Lijiao Ren;Yining Wu;N. Ren;Kun Zhang;D. Xing
为了研究反应器性能和微生物之间的相关性，一个集成的A/O反应器运行72天，处理稀释的畜禽废水。当进水COD和TN约为1000 mg/L时，COD去除率从79%提高到94%，总氮去除率从37%提高到50%（HRT为7.4h）。1500 mg/L和95 mg/L，最终出水COD浓度小于100 mg/L。启动过程中采用基于16 S rRNA基因的变性梯度凝胶电泳（DGGE）技术对微生物群落进行监测。DGGE图谱显示，在启动过程中，微生物群落发生了显着变化，这些变化与反应器的性能是一致的。UPGMA聚类分析表明，14个厌氧样品可分为5个主要类群，好氧样品也可分为5个主要类群，但聚类模式不同。系统发育分析表明，厌氧区的微生物群属于厚壁菌门（Firmicutes）、变形菌门（Proteobacteria）、绿弯菌门（Chloroflexi）和拟杆菌门（Bacteroidetes），而好氧区的微生物群则属于变形菌门（Proteobacteria）、拟杆菌门（Bacteroidetes）、厚壁菌门（Firmicutes）、疣微生物门（Verrucomicrobacteria）和硝化螺旋菌门（Nitrospira）。在厌氧区，在启动过程中检测到更多的发酵和产乙酸细菌，而厌氧细菌逐渐消失。当脱氮率较高时，可观察到两个功能种群，如德氏硝化螺菌和嗜酸脱氯单胞菌，表明在好氧隔室中发生了同步硝化反硝化作用。
In order to investigate the correlation between reactor performance and the microorganisms, an integrated A/O reactor was operated for 72 days to treat diluted livestock wastewater. Chemical oxygen demand (COD) removal efficiency increased from 79% to 94%, with total nitrogen (TN) removal efficiency from 37% to 50% (HRT 7.4 hr) when the influent COD and TN were ca. 1500 mg/L and 95 mg/L, respectively, and the outlet COD concentration was less than 100 mg/L at the end. Microbial community was monitored during start-up period by denaturing gradient gel electrophoresis (DGGE) based on 16S rRNA gene. DGGE profiles showed that microbial community had changed significantly during the start-up and these shifts were in accordance with the reactor performance. UPGMA clustering analysis showed that 14 anaerobic samples fell into five main groups and so did the aerobic ones, but the grouping patterns were different. Phylogenetic analysis indicated that microbial populations in the anaerobic compartment belonged to Firmicutes, Proteobacteria, Chloroflexi and Bacteroidetes, while Proteobacteria, Bacteroidetes, Firmicutes, Verrucomicrobiae and Nitrospira were present in the aerobic compartment. In the anaerobic compartment, more fermentative and acetogenic bacteria were detected during the start-up while denitrifying bacteria faded away. Two functional populations such as Nitrospira defluvii and Dechloromonas denitrificans were observed when nitrogen removal was high, indicating that simultaneous nitrification and denitrification occurred in the aerobic compartment.