Fully Automated Evaluation of Total Glomerular Number and Capillary Tuft Size in Nephritic Kidneys Using Lightsheet Microscopy
Fully Automated Evaluation of Total Glomerular Number and Capillary Tuft Size in Nephritic Kidneys Using Lightsheet Microscopy
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DOI：
10.1681/asn.2016020232
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发表时间：
2017-02-01
影响因子：
13.6
通讯作者：
Gunzer, Matthias
中科院分区：
文献类型：
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作者：
Klingberg, Anika;Hasenberg, Anja;Gunzer, Matthias
肾小球总数是肾功能的基本参数，但使用标准方法很难确定。在这里，我们对小鼠肾脏中的所有单个肾小球进行了计数，并通过结合体内内皮细胞荧光标记、一种新型组织透明技术、光片显微镜和图像分析自动配准来确定毛细血管簇的大小。每个器官的总动手时间是
The total number of glomeruli is a fundamental parameter of kidney function but very difficult to determine using standard methodology. Here, we counted all individual glomeruli in murine kidneys and sized the capillary tufts by combining in vivo fluorescence labeling of endothelial cells, a novel tissue-clearing technique, lightsheet microscopy, and automated registration by image analysis. Total hands-on time per organ was