Methylobacterium planium sp. nov., isolated from a lichen sample
Methylobacterium planium sp. nov., isolated from a lichen sample
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DOI：
10.1007/s00203-020-01881-4
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发表时间：
2020-04-18
影响因子：
2.8
通讯作者：
Jiang, Yi
中科院分区：
文献类型：
--
作者：
Jiang, Longqian;An, Defeng;Jiang, Yi
从采自云南省的麻风菌地衣中分离到一株新的细菌，命名为YIM 132548（T）。该微生物为革兰氏染色阴性、需氧和甲基营养型。细胞过氧化氢酶阳性，氧化酶阴性，无孢子，杆状，有三根极生鞭毛，能运动。该菌株可在15-30 ℃（最适，20 ℃）、pH 6.0-9.0（最适，pH 7.0）下生长，并且在NaCl存在下不生长。16 S rRNA基因序列分析表明，菌株YIM 132548（T）与Methylobacterium soli YIM 48816（T）和Methylobacterium durans NBRC 112876（T）的16 S rRNA基因序列相似性较高（分别为97.6%和97.3%），而与其它已命名的甲基杆菌属模式菌株的相似性低于97.0%。泛醌Q-10是主要的呼吸泛醌。主要的细胞脂肪酸被鉴定为总特征8（C-18：1 ω 7 c）。主要极性脂质为二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油和磷脂酰胆碱。基因组序列草图的DNA G + C含量为70.2mol%。YIM 132548（T）与M. soli YIM 48816（T）和M.与durans NBRC 112876（T）的同源性分别为87.0%和82.0%、40.6%和27.2%。基于系统发育、化学分类、表型和基因组数据，YIM 132548（T）菌株被认为是甲基杆菌属的一个新种，命名为扁平甲基杆菌（Methylobacterium planium sp. nov.）。模式菌株为YIM 132548（T）（= CGMCC 1.17323（T）= NBRC 114056（T））。
A novel bacterial strain, designated YIM 132548( T), was isolated from Lepraria sp. lichen collected from Yunnan province, south-west PR China. The organism was Gram-stain negative, aerobic and methylotrophic. The cell was catalase positive and oxidase negative, asporogenous, rod-shaped and motile with three polar flagella. The strain could grow at 15-30 degrees C (optimum, 20 degrees C), at pH 6.0-9.0 (optimum, pH 7.0) and does not grow in the presence of NaCl. According to the 16S rRNA gene sequence analysis, strain YIM 132548( T) showed high levels of 16S rRNA gene sequence similarity with Methylobacterium soli YIM 48816( T) (97.6%) and Methylobacterium durans NBRC 112876( T) (97.3%), less than 97.0% with other validly named type strains of the genus Methylobacterium. Ubiquinone Q-10 was the predominant respiratory ubiquinone. The predominant cellular fatty acid was identified as summed feature 8 (C-18:1 omega 7c). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The DNA G + C content of the draft genome sequence is 70.2 mol%. The average nucleotide identity and digital DNA-DNA hybridizations values of strain YIM 132548( T) with M. soli YIM 48816( T) and M. durans NBRC 112876( T) were 87.0% and 82.0%, 40.6% and 27.2% based on draft genome sequences, respectively. On the basis of phylogenetic, chemotaxonomic, phenotypic and genomic data, strain YIM 132548( T) is concluded to represent a novel species of the genus Methylobacterium, for which the name Methylobacterium planium sp. nov. is proposed. The type strain is YIM 132548( T) (= CGMCC 1.17323( T) = NBRC 114056( T)).