Chemical unfolding of protein domains induces shape change in programmed protein hydrogels
Chemical unfolding of protein domains induces shape change in programmed protein hydrogels
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DOI：
10.1038/s41467-019-13312-0
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发表时间：
2019-11-29
影响因子：
16.6
通讯作者：
Popa, Ionel
中科院分区：
文献类型：
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作者：
Khoury, Luai R.;Popa, Ionel
可编程行为结合定制刚度和可调生物力学响应是开发成功材料的关键要求。然而，这些特性对于蛋白质基生物材料来说仍然是一个难以捉摸的目标。在这里，我们使用蛋白质-聚合物的相互作用，以操纵由牛血清白蛋白（BSA）制成的蛋白质为基础的水凝胶的刚度，通过使用聚电解质，如聚乙烯亚胺（PEI）和聚-L-赖氨酸（PLL）在各种浓度。这种方法赋予蛋白质-水凝胶可调的宽范围刚度，从类似的10-64 kPa，而不影响蛋白质力学和纳米结构。我们使用PEI诱导的刚度增加6倍来编程各种形状的BSA水凝胶。利用蛋白质的去折叠特性，我们可以用化学变性溶液诱导这些复合材料的可逆形状记忆行为。这里展示的方法，基于蛋白质工程和聚合物增强，可以使智能生物材料的开发和研究，并扩展蛋白质水凝胶的能力超出其传统的应用。
Programmable behavior combined with tailored stiffness and tunable biomechanical response are key requirements for developing successful materials. However, these properties are still an elusive goal for protein-based biomaterials. Here, we use protein-polymer interactions to manipulate the stiffness of protein-based hydrogels made from bovine serum albumin (BSA) by using polyelectrolytes such as polyethyleneimine (PEI) and poly-L-lysine (PLL) at various concentrations. This approach confers protein-hydrogels with tunable wide-range stiffness, from similar to 10-64 kPa, without affecting the protein mechanics and nanostructure. We use the 6-fold increase in stiffness induced by PEI to program BSA hydrogels in various shapes. By utilizing the characteristic protein unfolding we can induce reversible shape-memory behavior of these composite materials using chemical denaturing solutions. The approach demonstrated here, based on protein engineering and polymer reinforcing, may enable the development and investigation of smart biomaterials and extend protein hydrogel capabilities beyond their conventional applications.