A morphometric study on the longitudinal and lateral symmetry of the sural nerve in mature and aging female rats
A morphometric study on the longitudinal and lateral symmetry of the sural nerve in mature and aging female rats
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DOI：
10.1016/j.brainres.2008.05.055
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发表时间：
2008-07-30
期刊：
影响因子：
2.9
通讯作者：
Sassoli Fazan, Valeria Paula
中科院分区：
文献类型：
--
作者：
Jeronimo, Andre;Dominques Jeronimo, Claudia Alem;Sassoli Fazan, Valeria Paula
在实验模型中，衰老影响周围神经功能和再生，但很少有文献报道涉及年龄超过一年的动物。我们研究了老龄大鼠腓肠神经的形态学和形态计量学方面。分别处死360、640和720日龄雌性Wistar大鼠，制备左右腓肠神经近端和远端，进行光镜和计算机形态测量。在所有实验组中，在同一水平上，近端和远端节段之间或左右侧之间均未发现形态计量学差异。从360天到720天，没有观察到纤维和轴突尺寸的增加。同样，两组之间也没有观察到总有髓纤维数量的差异。有髓纤维群分布为双峰，720日龄动物的分布向左移动，表明纤维直径减小。720日龄动物的有髓纤维的比率分布也向左移动，这表明轴突萎缩。观察到由于老化引起的形态学改变，主要与髓鞘有关，这表明脱髓鞘。大纤维比小纤维受到的影响更大。轴突异常并不常见或明显的髓鞘的变化和沃勒变性是罕见的found.These改变都观察到在所有实验组，但在大鼠360天的年龄要不太明显，其严重程度随着年龄的增长。总之，本研究表明，雌性大鼠腓肠神经中存在的衰老性神经病变是轴突性和脱髓鞘性的。(C)2008 Elsevier B.V.保留所有权利。
Aging affects peripheral nerve function and regeneration in experimental models but few literature reports deal with animals aged more than one year. We investigated morphological and morphometric aspects of the sural nerve in aging rats. Female Wistar rats 360, 640 and 720 days old were killed, proximal and distal segments of the right and left sural nerves were prepared for light microscopy and computerized morphometry. No morphometric differences between proximal and distal segments or between right and left sides at the same levels were found in all experimental groups. No increase in fiber and axon sizes was observed from 360 to 720 days. Likewise, no difference in total myelinated fiber number was observed between groups. Myelinated fiber population distribution was bimodal, being the 720-days old animals' distribution shifted to the left, indicating a reduction of the fiber diameters. The 9 ratio distribution of the 720-days old animals' myelinated fiber was also shifted to the left, which suggests axonal atrophy. Morphological alterations due to aging were observed, mainly related to the myelin sheath, which suggests demyelination. Large fibers were more affected than the smaller ones. Axon abnormalities were not as common or as obvious as the myelin changes and Wallerian degeneration was rarely found. These alterations were observed in all experimental groups but were much less pronounced in rats 360 days old and their severity increased with aging. in conclusion, the present study indicates that the aging neuropathy present in the sural nerve of female rats is both axonal and demyelinating. (C) 2008 Elsevier B.V. All rights reserved.