The isolation of tetrahydroxy bile acids as methyl esters from human urine and their characterization by 1H- and 13C-nuclear magnetic resonance spectroscopy.
The isolation of tetrahydroxy bile acids as methyl esters from human urine and their characterization by 1H- and 13C-nuclear magnetic resonance spectroscopy.
复制标题
从人尿中分离四羟基胆汁酸甲酯，并通过 1H 和 13C 核磁共振波谱对其进行表征。
DOI：
10.1515/bchm2.1984.365.1.479
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
C. Populoh
中科院分区：
文献类型：
--
作者：
P. Back;H. Fritz;C. Populoh
苯巴比妥治疗引起肝内胆汁淤积症患者四羟基化胆汁酸尿排泄增加。通过柱色谱和薄层色谱法从尿液中分离出主要成分，并通过核磁共振光谱法研究其甲酯。所得结果强烈支持以下论点：主要成分为胆酸的1 β-、6 α-和6 β-羟基化衍生物。
Treatment with phenobarbital causes an increased urinary excretion of tetrahydroxylated bile acids in patients suffering from intrahepatic cholestasis. The main components were isolated from urine by means of column and thin-layer chromatography and were studied as methyl esters by nuclear magnetic resonance spectroscopy. The results obtained strongly support the contention that the main components are 1 beta-, 6 alpha- and 6 beta-hydroxylated derivatives of cholic acid.