Electrical pulse stimulation-induced muscle contraction alters the microRNA and mRNA profiles of circulating extracellular vesicles in mice.
Electrical pulse stimulation-induced muscle contraction alters the microRNA and mRNA profiles of circulating extracellular vesicles in mice.
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电脉冲刺激诱导的肌肉收缩改变了小鼠循环细胞外囊泡的 microRNA 和 mRNA 谱。
DOI：
10.1152/ajpregu.00121.2022
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Suzuki Katsuhiko.
中科院分区：
文献类型：
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作者：
Kawanihi Noriaki;Tominaga Takaki;Suzuki Katsuhiko.
细胞外囊泡（例如外泌体）由骨骼肌组织分泌，可能在运动诱导的生理适应中发挥作用。耐力运动改变了循环细胞外囊泡的microRNA（miRNA）谱;然而，阻力运动的影响尚不清楚。在这项研究中，我们使用一种全面的基于RNA测序的方法，研究了抗阻运动作为电脉冲刺激（EPS）诱导的肌肉收缩对小鼠循环细胞外囊泡的miRNA和mRNA谱的影响。EPS诱导的肌肉收缩导致循环细胞外囊泡的miRNA谱的变化。特别是，在EPS诱导的肌肉收缩后90分钟，观察到肌肉特异性微小RNA（例如miR-1、miR-133和miR-206）的表达显着增加。此外，我们发现，208 mRNA的表达显着改变后立即EPS诱导的肌肉收缩和267 mRNA的变化显着90分钟后。基因本体富集分析表明，在循环细胞外囊泡的mRNA表达变化后，EPS诱导的肌肉收缩促进血管生成和调节免疫反应。由于肌肉收缩引起的循环细胞外囊泡性质的变化可能在阻力运动诱导的生理适应中起重要作用。
Extracellular vesicles, such as exosomes, are secreted by skeletal muscle tissues and may play a role in physiological adaptations induced by exercise. Endurance exercise changes the microRNA (miRNA) profile of circulating extracellular vesicles; however, the effects of resistance exercise are unknown. In this study, we examined the effect of resistance exercise as electrical pulse stimulation (EPS)-induced muscle contraction on the miRNA and mRNA profiles of circulating extracellular vesicles in mice using a comprehensive RNA sequencing-based approach. EPS-induced muscle contraction resulted in changes in the miRNA profile of circulating extracellular vesicles. In particular, 90 min after EPS-induced muscle contraction, a considerable increase in expression of muscle-specific microRNAs, such as miR-1, miR-133, and miR-206, was observed. Furthermore, we found that the expression of 208 mRNAs was considerably altered immediately after EPS-induced muscle contraction and that of 267 mRNAs changed considerably after 90 min. Gene ontology enrichment analysis showed that mRNA expression changes in circulating extracellular vesicles after EPS-induced muscle contraction promoted angiogenesis and regulated the immune response. Changes in the properties of circulating extracellular vesicles owing to muscle contraction may play an important role in resistance exercise-induced physiological adaptations.