Transcription expression and clinical significance of dishevelled-3 mRNA and δ-catenin mRNA in pleural effusions from patients with lung cancer.
Transcription expression and clinical significance of dishevelled-3 mRNA and δ-catenin mRNA in pleural effusions from patients with lung cancer.
复制标题
DOI：
10.1155/2012/904946
复制
发表时间：
2012
影响因子：
--
通讯作者：
Wu GP
中科院分区：
文献类型：
--
作者：
Li XY;Liu SL;Cha N;Zhao YJ;Wang SC;Li WN;Wang EH;Wu GP
Objective.目的探讨胸水Dishevelled-3（DVL-3）mRNA和δ-catenin mRNA表达对肺癌的诊断价值。方法.通过对来自肺癌患者（n = 75）和肺良性疾病患者（n = 51）的胸腔积液标本进行RT-PCR来评估DVL-3 mRNA和δ-连环蛋白mRNA水平。结果DVL-3 mRNA和δ-catenin mRNA在肺恶性病变中的表达明显高于肺良性病变（P < 0.01），在肺腺癌中的表达明显高于细胞学检查（P < 0.01）。在单次使用中，DVL-3 mRNA的特异性最高（94.1%），PPV最高（95.7%），而δ-catenin mRNA的敏感性最高（92.0%），NPV最高（88.5%）。当同时评估标记物的组合时，DVL-3 mRNA和δ-catenin mRNA给出了高诊断性能：灵敏度为100.0%，NPV为100.0%，准确度为96.0%。结论DVL-3 mRNA和δ-catenin mRNA作为检测胸腔微转移的分子标志物，有助于肺癌患者胸腔积液中癌细胞的诊断。
Objective. To evaluate diagnostic utility of Dishevelled-3 (DVL-3) mRNA and δ-catenin mRNA expression in pleural effusions of patients with lung cancer. Methods. DVL-3 mRNA and δ-catenin mRNA levels were assessed by performing RT-PCR on pleural effusion specimens from patients with lung cancer (n = 75) and with lung benign disease (n = 51). Results. The expressions of DVL-3 mRNA and δ-catenin mRNA were significantly higher in malignant than in benign lung disease (P < 0.01) and were obviously higher than cytology in adenocarcinoma (P < 0.01). In single use, DVL-3 mRNA had the highest specificity (94.1%) and PPV (95.7%), whereas δ-catenin mRNA had the highest sensitivity (92.0%) and NPV (88.5%). When combinations of markers were evaluated together, DVL-3 mRNA and δ-catenin mRNA gave a high-diagnostic performance: sensitivity of 100.0%, NPV of 100.0%, and accuracy of 96.0%, respectively. Conclusion. As molecular markers of detecting pleural micrometastasis, DVL-3 mRNA and δ-catenin mRNA are helpful to diagnose the cancer cells in pleural effusions of patients with lung cancer.