Analyses of Cullin1 homologs reveal functional redundancy in S-RNase-based self-incompatibility and evolutionary relationships in eudicots
Analyses of Cullin1 homologs reveal functional redundancy in S-RNase-based self-incompatibility and evolutionary relationships in eudicots
复制标题
Cullin1 同源物的分析揭示了真双子叶植物中基于 S-RNase 的自交不亲和性和进化关系的功能冗余
DOI：
10.1093/plcell/koac357
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Kao, Teh-hui
中科院分区：
文献类型：
--
作者：
Sun, Linhan;Cao, Shiyun;Zheng, Ning;Kao, Teh-hui
矮牵牛自交不亲和性（self-incompatibility，SI）受一个多态性的S位点调控，该位点含有雌蕊特异的S-RNase和多个花粉特异的S-Locus F-box（SLF）基因。SLF组装成E3遍在蛋白连接酶复合物，称为Skp 1-Cullin 1-F-box复合物（SCFSLF）。在花粉管中，这些复合物共同介导所有非自身S-RNase的泛素化和降解，但不是自身S-RNase，导致交叉亲和，但自交不亲和，授粉。利用矮牵牛，我们发现两个花粉表达的Cullin 1（CUL 1）蛋白，PiCUL 1-P和PiCUL 1-B，在SI中冗余功能。这种冗余在矮牵牛中丢失，不是因为PhCUL 1-B不能与SSK 1相互作用，而是由于PhCUL 1-B转录水平的降低。这可能是由于在PhCUL 1-B启动子区域存在DNA转座子，该转座子遗传自Pe的亲本种之一的矮牵牛。杂种。Cullingenes在不同真双子叶植物中的系统发育和同线分析表明，3个茄科特有的CUL 1基因具有共同的起源，CUL 1-P致力于S-RNase相关的生殖过程。然而，CUL 1-B是CUL 1-P的一个分散的重复，仅存在于矮牵牛中，而不存在于所研究的茄科的其他物种中。我们认为，thecul 1参与（或可能参与）在真双子叶植物的SI响应共享一个共同的起源。
InPetunia(Solanaceae family), self-incompatibility (SI) is regulated by the polymorphicS-locus, which contains the pistil-specificS-RNaseand multiple pollen-specificS-Locus F-box(SLF) genes. SLFs assemble into E3 ubiquitin ligase complexes known as Skp1–Cullin1–F-box complexes (SCFSLF). In pollen tubes, these complexes collectively mediate ubiquitination and degradation of all nonself S-RNases, but not self S-RNase, resulting in cross-compatible, but self-incompatible, pollination. UsingPetunia inflata, we show that two pollen-expressed Cullin1 (CUL1) proteins, PiCUL1-P and PiCUL1-B, function redundantly in SI. This redundancy is lost inPetunia hybrida, not because of the inability of PhCUL1-B to interact with SSK1, but due to a reduction in thePhCUL1-Btranscript level. This is possibly caused by the presence of a DNA transposon in thePhCUL1-Bpromoter region, which was inherited fromPetunia axillaris, one of the parental species ofPe. hybrida. Phylogenetic and syntenic analyses ofCullingenes in various eudicots show that three Solanaceae-specificCUL1genes share a common origin, withCUL1-Pdedicated to S-RNase-related reproductive processes. However,CUL1-Bis a dispersed duplicate ofCUL1-Ppresent only inPetunia, and not in the other species of the Solanaceae family examined. We suggest that theCUL1s involved (or potentially involved) in the SI response in eudicots share a common origin.