Antimicrobial resistance and molecular characteristics of Neisseria gonorrhoeae isolates from men who have sex with men
Antimicrobial resistance and molecular characteristics of Neisseria gonorrhoeae isolates from men who have sex with men
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DOI：
10.1016/j.ijid.2018.10.030
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发表时间：
2019-02-01
影响因子：
8.4
通讯作者：
Pereira, Filomena
中科院分区：
文献类型：
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作者：
Calado, Joana;Castro, Rita;Pereira, Filomena
目的：目的分析淋病奈瑟菌对青霉素（Pen）、头孢克肟（Cfm）、头孢曲松（Cro）、四环素（泰特）、环丙沙星（Cip）、阿奇霉素（Azm）、壮观霉素（Spt）的敏感性，并验证耐药基因是否存在突变。淋病分离株收集自MSM（男男性行为者）人群。通过PCR和DNA测序鉴定中度耐药和完全耐药菌株中佩纳、mtrR、gyrA和parC基因的突变。淋病分离株对Pen（73%）、Cfm（3%）、泰特（60%）、Cip（37%）和Azm（13%）表现出中等或完全耐药。一个分离与耐Cfm提出了青霉素结合蛋白2（PBP 2）马赛克XXXIV。所有对Pen具有中等或完全抗性的菌株（PBP 2嵌合体除外）在PBP 2中呈现D345 a。所有Cip耐药分离株在gyrA基因中具有S91 F，同时在parC基因中具有突变。所有对MtrCDE外排泵底物具有中间或完全抗性的分离株在mtrR基因中具有A39 T或G45 D突变或在mtrR启动子内具有腺嘌呤缺失。一个分离提出了脑膜炎奈瑟氏球菌样mtrR启动子sequence.Conclusions：这项研究的结果是一致的，与其他研究的结果，并加强了新的治疗方案的权宜发展的重要性。(c)2018作者由Elsevier Ltd代表国际传染病学会出版。这是一个在CC BY-NC-ND许可证下的开放获取文章（http：//creativecommons.org/licenses/by-nc-nd/4.0/）。
Objectives: To analyze the susceptibility of Neisseria gonorrhoeae isolates to penicillin (Pen), cefixime (Cfm), ceftriaxone (Cro), tetracycline (Tet), ciprofloxacin (Cip), azithromycin (Azm), and spectinomycin (Spt), and to verify the presence of mutations in resistance genes.Methods: Antibiotic susceptibility testing was performed by Etest method on 30 N. gonorrhoeae isolates collected from the MSM (men who have sex with men) population. PCR and DNA sequencing were performed to identify mutations within the penA, mtrR, gyrA, and parC genes in intermediately resistant and fully resistant isolates.Results: N. gonorrhoeae isolates showed intermediate or full resistance to Pen (73%), Cfm (3%), Tet (60%), Cip (37%), and Azm (13%). One isolate with resistance to Cfm presented a penicillin-binding protein 2 (PBP2) mosaic XXXIV. All isolates with intermediate or full resistance to Pen (except at PBP2 mosaic) presented a D345a in PBP2. All Cip-resistant isolates had an S91F in the gyrA gene together with mutations in the parC gene. All intermediate or fully resistant isolates to substrates of the MtrCDE efflux pump had an A39T or G45D mutation in the mtrR gene or an adenine deletion within the mtrR promoter. One isolate presented a Neisseria meningitidis-like mtrR promoter sequence.Conclusions: The results of this study are consistent with the findings of other studies and reinforce the importance of the expedient development of new therapeutic options. (c) 2018 The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).