Decrease of immature B cell and interleukin-10 during early-post-transplant period in renal transplant recipients under tacrolimus based immunosuppression
Decrease of immature B cell and interleukin-10 during early-post-transplant period in renal transplant recipients under tacrolimus based immunosuppression
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DOI：
10.1016/j.trim.2014.03.003
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发表时间：
2014-05-01
影响因子：
1.5
通讯作者：
Yang, Chul Woo
中科院分区：
文献类型：
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作者：
Chung, Byung Ha;Kim, Kyoung Woon;Yang, Chul Woo
已知B淋巴细胞在肾移植(KT)的结果中起作用。在这里，我们评估了KT前后B细胞亚群的比例。21例同种异体肾移植患者在术后一个月内无急性排斥反应，移植肾功能稳定，纳入本研究。分别于移植前和移植后1个月采集外周血标本。用多色流式细胞术检测移植后B细胞亚群比例的变化。外周血单个核细胞(PBMC)中淋巴细胞和CD19(+)B细胞占白细胞总数的比例在KT后无明显变化。同样，CD19(+)CD24(+)淋巴细胞、成熟B细胞(CD24(Int)CD38(Inter)/CD19(+))和记忆性B细胞(CD24(+)CD38(-)/CD19(+))的比例在KT后没有变化。而未成熟B细胞(CD24(+)、CD38(+)/CD19(+)B细胞)的比例在移植后明显下降(P
B lymphocytes are known to play a role in kidney transplantation (KT) outcomes. Here, we evaluated the proportion of B cell subsets before and after KT. Twenty-one patients, who showed stable allograft function without acute rejection in the month following the KT, were included in this study. Peripheral blood samples were obtained from these patients before transplantation as well as 1 month after transplantation. Changes in the proportion of B cell subsets after transplantation were investigated using multi-color flow cytometry. The proportion of lymphocytes in the peripheral blood mononuclear cells (PBMCs) and of CD19(+) B cells in the total leukocyte population did not change after KT. Similarly, the proportions of CD19(+)CD24(+) lymphocytes, mature B cells (CD24(Int)CD38(Inter)/CD19(+)) and memory B cells (CD24(+)CD38(-)/CD19(+)) did not change post-KT. However, the proportion of immature B cells (CD24(+)CD38(+)/CD19(+) B cells) decreased significantly after transplantation (P