A Quantitative, Clonal Assay for Carcinogen-Induced Alterations of Respiratory Epithelial Cells in Culture
A Quantitative, Clonal Assay for Carcinogen-Induced Alterations of Respiratory Epithelial Cells in Culture
复制标题
培养物中致癌物诱导的呼吸道上皮细胞改变的定量克隆测定
DOI：
10.1007/978-1-4613-3611-2_23
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发表时间：
1983
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影响因子：
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通讯作者：
D. Thomassen
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作者：
J. Barrett;T. Gray;M. Mass;D. Thomassen
环境致癌物的识别和这些物质对人类的潜在风险的评估需要实验系统来定量测量致癌物和促进剂的活性。细胞培养系统是此类研究的潜在非常有用的实验模型。短期，廉价的细胞培养致癌物质的测定是可用的。这些测定具有高的预测能力，用于检测已知的致癌物，具有很少的假阳性结果（Barrett等人，1980年）。测量的终点，即受试细胞的癌前或肿瘤转化，与致癌过程相关，而不是基于理论相关性。这些系统也可用于肿瘤发展的细胞和分子基础的机制研究。不同物种（包括人类）的细胞和组织的结果可以在相似的实验条件下进行比较和对比。
The identification of environmental carcinogens and the assessment of the potential risks of these substances to humans require experimental systems to measure quantitatively the activity of carcinogens and promoters. Cell culture systems are potentially very useful experimental models for such studies. Short-term, inexpensive cell culture assays for carcinogens are available. These assays have a high predictive ability for the detection of known carcinogens with few false positive results (Barrett et al., 1980). The end point measured, preneoplastic or neoplastic transformation of the test cells, is relevant to the carcinogenic process and is not predicated on a theoretical correlation. These systems can also be used for mechanistic studies on the cellular and molecular basis of neoplastic development. The results with cells and tissues of different species (including human) can be compared and contrasted under similar experimental conditions.
Fujiwara,Y.：江恩癌症研究专着。
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