Research advance and applications in aquatic metagenomics
Research advance and applications in aquatic metagenomics
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发表时间：
2010
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通讯作者：
Shen Lin
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作者：
Shen Lin
微生物是湖泊生态系统中最大的基因资源，是生物圈过程的主要驱动力。然而，由于目前环境群落中的绝大多数微生物无法通过常规富集方法进行培养，人们对水体微生物群落的结构和功能知之甚少，这给湖泊生物修复方法的应用带来了障碍。最近出现的宏基因组学方法是一种很有前景的方法。 一方面，宏基因组学可以作为开发生物多样性的工具，恢复湖泊微生物群落的代谢途径，为污染水的控制提供基础知识。另一方面，酶，特别是新型酶在许多行业中提供了巨大的潜力。近年来，宏基因组学在克服许多障碍的情况下取得了很大的进展。 阐述了湖泊生态系统宏基因组学的概念、方法、应用和挑战。
Microorganisms represent the largest gene resources and are the major drivers of biosphere processes in lake ecosystem.However,due to the fact that the vast majority of microbes in environmental communities cannot be cultured by conventional enrichment method until now,little is known about the structure and function of the water body microbial community,which forms a barrier in using bioremediation approaches in lakes.The recently emerged approach metagenomics is a promising technology independent of culture and offers a new way to conquer this problem.On one hand,metagenomics could be used as a tool to exploit the biodiversity,recover the metabolic pathway of lake microbial communities,which provide basic knowledge for polluted water control.On the other hand,enzyme,especially the novel enzyme provided great potential in many industries.In the latest years,metagenomics achieved much with many barriers existing.In the present review,the concept,methodology,application and challenges of metagenomics in lake ecosystem were described.