Residues of nitrofuran antibiotic parent compounds and metabolites in eyes of broiler chickens
Residues of nitrofuran antibiotic parent compounds and metabolites in eyes of broiler chickens
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DOI：
10.1080/02652030701586657
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发表时间：
2008-05-01
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影响因子：
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通讯作者：
Kennedy, D. G.
中科院分区：
文献类型：
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作者：
Cooper, K. M.;Mccrack-En, R. J.;Kennedy, D. G.
食品中的硝基呋喃抗生素残留仍然是国际关注的问题。发现氨基脲（SEM）的来源，而不是通过滥用呋喃西林，对使用SEM作为该药物的确定性标记残留物提出了挑战。检测完整的（母体）呋喃西林可避免混淆SEM残留物的来源。肉鸡饲喂亚治疗含硝基呋喃的饮食，并通过液相色谱-串联质谱检测（LC-MS/MS）分析其组织中的母体化合物和代谢物。组织结合代谢物残留物在肌肉中的消除半衰期为3.4天-3-氨基-2-恶唑烷酮（AOZ）、3-氨基-5-吗啉甲基-2-恶唑烷酮（AMOZ）-4.5天（SEM），而总代谢物残留物的消除半衰期为2.0天（AOZ）-3.2天（SEM）。眼睛中的代谢物浓度高于肌肉。眼睛中的代谢物半衰期范围为8.5天（1-氨基乙内酰脲（AHD））至20.3天（SEM）。在眼睛中也检测到硝基呋喃母体化合物。在停用6 mg kg（-1）呋喃他酮饮食21天后，在单眼中检测到呋喃他酮。当50只来自肌肉中含有接近1 μ g kg（-1）最低要求性能水平（MRPL）的代谢物的肉鸡眼睛合并成单个样品时，1.检测到呋喃唑酮2ng和呋喃他酮31.1ng，但由于SEM在肌肉中的耗尽半衰期长，未检测到呋喃西林。需要进一步的研究，以提高LC-MS/MS呋喃西林的灵敏度，并细化样本量所需的使用呋喃西林检测在合并的眼睛作为补充扫描电镜检测肌肉。
Nitrofuran antibiotic residues in food continue to be of international concern. The finding of sources of semicarbazide (SEM), other than through the misuse of nitrofurazone, present a challenge to the use of SEM as a definitive marker residue for this drug. Detection of intact (parent) nitrofurazone would avoid confusion over the source of SEM residues. Broiler chickens were fed sub-therapeutic nitrofuran-containing diets and their tissues were analysed for parent compounds and metabolites by liquid chromatography coupled with tandem mass spectrometry detection (LC-MS/MS). Depletion half-lives in muscle were longer for tissue-bound metabolite residues, 3.4 days -3-amino-2-oxazolidinone (AOZ), 3-amino-5-morpholinomethyl-2-oxazolidone (AMOZ) - to 4.5 days (SEM), than total metabolite residues, 2.0 days (AOZ) to 3.2 days (SEM). Metabolite concentrations were higher in eyes than in muscle. Metabolite half-lives in eyes ranged from 8.5 days (1-aminohydantoin (AHD)) to 20.3 days (SEM). Nitrofuran parent compounds were also detected in eyes. Furaltadone was detected in single eyes after 21 days' withdrawal of a 6 mgkg(-1) furaltadone diet. When 50 eyes from broilers containing metabolites in muscle close to the 1 mu g kg(-1) minimum required performance level (MRPL) were pooled into single samples, 1. 2 ng of furazolidone and 3 1.1 ng of furaltadone were detected, but nitrofurazone was not detected due to the long depletion half-life of SEM in muscle. Further studies are required to improve LC-MS/MS nitrofurazone sensitivity and refine the sample size necessary to use nitrofurazone detection in pooled eyes as a complement to SEM detection in muscle.