Clinical and Pathologic Study of Feline Merkel Cell Carcinoma With Immunohistochemical Characterization of Normal and Neoplastic Merkel Cells
Clinical and Pathologic Study of Feline Merkel Cell Carcinoma With Immunohistochemical Characterization of Normal and Neoplastic Merkel Cells
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DOI：
10.1177/0300985815570484
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发表时间：
2015-11-01
影响因子：
2.4
通讯作者：
Nakayama, H.
中科院分区：
文献类型：
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作者：
Dohata, A.;Chambers, J. K.;Nakayama, H.
作者在此描述了正常默克尔细胞的形态学和免疫组织化学特征以及猫默克尔细胞癌的临床病理学发现。默克尔细胞的特征是空泡透明细胞，并且单独位于所有检查区域的表皮基底层中。经常在面部和腕骨的窦毛附近观察到默克尔细胞簇。免疫组织化学显示，默克尔细胞的细胞角蛋白 (CK) 20、CK18、p63、神经元特异性烯醇化酶、突触素和蛋白质基因产物 9.5 呈阳性。在 3 只老年猫（13 至 16 岁）中检测到默克尔细胞癌，呈孤立性皮肤肿块。细胞学检查，所有病例均观察到大淋巴细胞样细胞。对手术切除的肿瘤进行组织学检查，发现圆形细胞巢被不同数量的纤维基质隔开。肿瘤细胞通常呈 CK20、CK18、p63、神经元特异性烯醇化酶和突触素免疫阳性，代表正常 Merkel 细胞的特征。
The authors herein describe the morphologic and immunohistochemical features of normal Merkel cells as well as the clinicopathologic findings of Merkel cell carcinoma in cats. Merkel cells were characterized as vacuolated clear cells and were individually located in the epidermal basal layer of all regions examined. Clusters of Merkel cells were often observed adjacent to the sinus hair of the face and carpus. Immunohistochemically, Merkel cells were positive for cytokeratin (CK) 20, CK18, p63, neuron-specific enolase, synaptophysin, and protein gene product 9.5. Merkel cell carcinoma was detected as a solitary cutaneous mass in 3 aged cats (13 to 16 years old). On cytology, large lymphocyte-like cells were observed in all cases. Histologic examinations of surgically resected tumors revealed nests of round cells separated by various amounts of a fibrous stroma. Tumor cells were commonly immunopositive for CK20, CK18, p63, neuron-specific enolase, and synaptophysin, representing the characteristics of normal Merkel cells.