Developmental competence of somatic cell nuclear transfer embryos reconstructed from oocytes matured in vitro with follicle shells in miniature pig
Developmental competence of somatic cell nuclear transfer embryos reconstructed from oocytes matured in vitro with follicle shells in miniature pig
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DOI：
10.1089/clo.2005.7.17
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发表时间：
2005-03-01
期刊：
影响因子：
--
通讯作者：
Imai, H
中科院分区：
文献类型：
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作者：
Hoshino, Y;Uchida, M;Imai, H
对转移至小型猪体细胞核的家猪卵母细胞的发育能力进行了检查。卵母细胞与卵泡壳的共培养系统用于家猪卵母细胞的体外成熟。共培养的卵母细胞比非共培养的卵母细胞更快、更同步地进入减数分裂中期 II 阶段。成熟48小时时，将卵母细胞去核并与Potbelly小型猪的成纤维细胞融合。使用共培养卵母细胞的核移植（NT）胚胎的囊胚形成率（24%）显着高于非共培养卵母细胞（13%）（p < 0.05）。使用共培养卵母细胞进行核移植48小时后裂解的胚胎被转移到14头哥廷根小型猪和4头梅山猪的输卵管中。所有哥廷根的发情期在怀孕20-31天左右恢复。梅山四人中有两人怀孕了。经过适度数量的胚胎移植（移植了 15 个和 29 个胚胎）后，分别诞生了三只和两只克隆仔猪。这些结果表明，与卵泡壳共培养的卵母细胞在核移植后具有较高的发育能力，并且在胚胎移植后可实现足月发育。
The developmental competence of domestic pig oocytes that were transferred to somatic cell nuclei of miniature pig was examined. A co-culture system of oocytes with follicle shells was used for the maturation of domestic pig oocytes in vitro. Co-cultured oocytes progressed to the metaphase II stage of meiosis more quickly and more synchronously than non co-cultured oocytes. Oocytes were enucleated and fused with fibroblast cells of Potbelly miniature pig at 48 h of maturation. The blastocyst formation rate of nuclear transfer (NT) embryos using co-cultured oocytes (24%) was significantly higher (p < 0.05) than that of non-co-cultured oocytes (13%). Cleaved embryos at 48 h after nuclear transfer using co-cultured oocytes were transferred to the oviducts of 14 Gottingen miniature pigs and four Meishan pigs. Estrus of all Gottingens returned at around 20-31 days of pregnancy. Two of the four Meishans became pregnant. Three and two cloned piglets were born after modest number of embryo transfer (15 and 29 embryos transferred), respectively. These results indicated that oocytes co-cultured with follicle shells have a high developmental competence after nuclear transfer and result in full-term development after embryo transfer.