The receptor-like kinase KLAVIER mediates systemic regulation of nodulation and non-symbiotic shoot development in Lotus japonicus
The receptor-like kinase KLAVIER mediates systemic regulation of nodulation and non-symbiotic shoot development in Lotus japonicus
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DOI：
10.1242/dev.058891
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发表时间：
2010-12-15
期刊：
影响因子：
4.6
通讯作者：
Kawaguchi, Masayoshi
中科院分区：
文献类型：
--
作者：
Miyazawa, Hikota;Oka-Kira, Erika;Kawaguchi, Masayoshi
在豆科植物中，共生根瘤的数量受茎与根之间的远距离交流控制。这种反馈机制缺陷的突变体表现出高调质表型。在这里，我们报道了一种新的富含亮氨酸的重复受体样激酶（LRR-RLK）， KLAVIER (KLV)，它介导了日本莲花结瘤的系统性负调控。在叶片中，KLV主要在维管组织中表达，另一个LRR-RLK基因HAR1也调节根瘤数量。双突变分析表明，KLV和HAR1在控制结瘤负调控的同一遗传途径中起作用。LjCLE-RS1和LjCLE-RS2代表了har1介导的根瘤系统调节的潜在根源性移动信号。LjCLE-RS1或LjCLE-RS2的过表达并未抑制klv突变体的高结瘤表型，这表明klv是必需的，并且在LjCLE-RS1和LjCLE-RS2的下游起作用。互补试验和表达分析表明，除了KLV在共生中的作用外，KLV在茎的发育过程中还发挥着多种作用，包括维持茎尖分生组织、维管的连续性、茎的生长和促进开花。在烟叶中瞬时表达的生化分析表明，KLV具有与HAR1和自身相互作用的能力。总之，这些结果表明，潜在的KLV- har1受体复合体调节共生结节的发育，KLV也是介导非共生芽发育的其他信号转导途径的关键成分。
In legumes, the number of symbiotic root nodules is controlled by long-distance communication between the shoot and the root. Mutants defective in this feedback mechanism exhibit a hypernodulating phenotype. Here, we report the identification of a novel leucine-rich repeat receptor-like kinase (LRR-RLK), KLAVIER (KLV), which mediates the systemic negative regulation of nodulation in Lotus japonicus. In leaf, KLV is predominantly expressed in the vascular tissues, as with another LRR-RLK gene, HAR1, which also regulates nodule number. A double-mutant analysis indicated that KLV and HAR1 function in the same genetic pathway that governs the negative regulation of nodulation. LjCLE-RS1 and LjCLE-RS2 represent potential root-derived mobile signals for the HAR1-mediated systemic regulation of nodulation. Overexpression of LjCLE-RS1 or LjCLE-RS2 did not suppress the hypernodulation phenotype of the klv mutant, indicating that KLV is required and acts downstream of LjCLE-RS1 and LjCLE-RS2. In addition to the role of KLV in symbiosis, complementation tests and expression analyses indicated that KLV plays multiple roles in shoot development, including maintenance of shoot apical meristem, vascular continuity, shoot growth and promotion of flowering. Biochemical analyses using transient expression in Nicotiana benthamiana revealed that KLV has the ability to interact with HAR1 and with itself. Together, these results suggest that the potential KLV-HAR1 receptor complex regulates symbiotic nodule development and that KLV is also a key component in other signal transduction pathways that mediate non-symbiotic shoot development.