Cerebral blood flow and apoptosis-associated factor with electroacupuncture in a traumatic brain injury rat model
Cerebral blood flow and apoptosis-associated factor with electroacupuncture in a traumatic brain injury rat model
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DOI：
10.1136/acupmed-2013-010406
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发表时间：
2013-12-01
影响因子：
2.5
通讯作者：
Kuo, Jinn Rung
中科院分区：
文献类型：
--
作者：
Chuang, Chih Hsiang;Hsu, Yao Chin;Kuo, Jinn Rung
目的电针已广泛应用于脑卒中的治疗，但电针对创伤性脑损伤（TBI）后神经保护作用的研究较少。方法雄性SD大鼠随机分为4组：假手术组、TBI对照组、TBI+EA 30 min组和TBI+EA 60 min组。TBI后立即用EA治疗动物。电针组采用疏密波，频率0.2和1Hz，幅度1 mA，电针穴位GV 20、GV 26、LI 4和KI 1，持续30或60 min。观察局部血流量、细胞梗死体积、神经元凋亡程度、细胞凋亡相关因子转化生长相互作用因子（TGIF）表达，并通过跑速试验评价功能结局。结果TBI后即刻，与对照组比较，电针60 min组脑局部血流量明显增加（P < 0. 05），电针60 min组脑局部血流量明显增加（P < 0. 05）。与对照组比较，电针治疗后72 h脑梗死大鼠的运动速度、梗死体积、神经元凋亡及脑梗死相关因子TGIF表达均明显改善。结论电针治疗脑梗死急性期60 min可增加脑梗死局部血流量，降低脑梗死相关因子TGIF的表达，可能导致神经元凋亡和细胞梗死体积的减少，并且可能代表可能发生功能恢复的一种机制。
Objective Electroacupuncture (EA) has been widely used for treatment of stroke, but there is little information on the effect of EA on the neuroprotective function in traumatic brain injury (TBI). The aim of the present study was to investigate the protective effects and mechanisms of EA treatment in a TBI rat model.Methods Male Sprague-Dawley rats were randomly divided into four groups: sham operation, TBI control, TBI+EA treated for 30min or TBI+EA treated for 60min. The animals were treated with EA immediately after TBI. The EA was applied at acupuncture points GV20, GV26, LI4 and KI1 with a dense-dispersed wave, frequencies of 0.2 and 1Hz, and amplitude of 1mA for 30 or 60min. Regional blood flow, cell infarction volume, extent of neuronal apoptosis, expression of cell apoptosis-associated factor transforming growth-interacting factor (TGIF) were studied, and functional outcome was assessed by running speed test. All tests except regional blood flow were performed 72h after TBI onset.Results Immediately after TBI, compared with the TBI control groups, the regional blood flow was significantly increased by EA treatment for 60min. Compared with the TBI controls 72h after TBI, the TBI-induced run speed impairment, infarction volume, neuronal apoptosis and apoptosis-associated TGIF expression were significantly improved by EA treatment.Conclusions The treatment of TBI in the acute stage with EA for 60min could increase the regional blood flow and attenuate the levels of TGIF in the injured cortex, might lead to a decrease in neuronal apoptosis and cell infarction volume, and might represent one mechanism by which functional recovery may occur.