Loss of maspin is a negative prognostic factor for invasion and metastasis in oral squamous cell carcinoma.
Loss of maspin is a negative prognostic factor for invasion and metastasis in oral squamous cell carcinoma.
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DOI：
10.1111/j.1600-0714.2009.00762.x
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发表时间：
2009-07
期刊：
影响因子：
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通讯作者：
K. Yoshizawa;S. Nozaki;Ayako Okamune;H. Kitahara;Teruhisa Ohara;K. Kato;S. Kawashiri;E. Yamamoto
中科院分区：
文献类型：
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作者：
K. Yoshizawa;S. Nozaki;Ayako Okamune;H. Kitahara;Teruhisa Ohara;K. Kato;S. Kawashiri;E. Yamamoto
目的Maspin是一种分子量为42-kDa的蛋白质，属于丝氨酸蛋白酶抑制剂家族，具有肿瘤抑制作用。在这项研究中，我们研究了口腔鳞状细胞癌（OSCC）的临床病理结果和maspin表达之间的相互关系。方法采用免疫组织化学方法检测maspin在口腔鳞癌组织中的表达，71例口腔鳞癌中maspin阳性表达46例（64.8%）。我们还比较了口腔鳞癌病例的克隆病理学特征与maspin表达水平。此外，我们研究了maspin的表达在8个细胞系来自口腔鳞癌使用逆转录-聚合酶链反应（RT-PCR）和蛋白质印迹。结果maspin表达下降与胃癌的T分期（P < 0.01）、淋巴结转移（P < 0.0001）和浸润方式（P < 0.0001）有关。maspin阳性表达者预后较好（P < 0.001）。maspin的低表达也见于来源于4D级的细胞系中，其显示出比其他等级的OSCC更强的侵袭潜力。结论Maspin可能是判断口腔鳞癌进展的一个有用指标。
OBJECTIVE Maspin, a 42-kDa protein, belongs to the serpin family of protease inhibitors and is known to have tumor-suppressor function. In this study, we investigated the interrelationship between clinicopathologic findings and maspin expression in oral squamous cell carcinoma (OSCC). METHODS Using immunohistochemical techniques to examine the expression levels of maspin in OSCC, maspin expression in OSCC was detected in 46 (64.8%) of 71 cases. We also compared the clonicopathologic features of OSCC cases with maspin expression levels. Moreover, we examined expression of maspin in eight cell lines derived from OSCC using reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting. RESULTS There was a significant correlation between decreased maspin expression and T-category (P < 0.01), lymph metastasis (P < 0.0001), and mode of invasion (P < 0.0001). Patients with positive maspin expression had a significantly better prognosis (P < 0.001). Lower expression of maspin was also seen in cell lines derived from grade 4D, which shows stronger invasive potential than other grades of OSCC. CONCLUSION Maspin may be a useful marker to identify the potential for progression in OSCC.