Physiological and transcriptomic analyses reveal a response mechanism to cold stress in Santalum album L. leaves.
Physiological and transcriptomic analyses reveal a response mechanism to cold stress in Santalum album L. leaves.
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生理和转录组分析揭示了檀香叶对冷应激的反应机制。
DOI：
10.1038/srep42165
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发表时间：
2017-02-07
影响因子：
4.6
通讯作者：
Ma G
中科院分区：
文献类型：
--
作者：
Zhang X;Teixeira da Silva JA;Niu M;Li M;He C;Zhao J;Zeng S;Duan J;Ma G
印度檀香（Santalum album L.）是一种经济上重要的植物物种，因为它能够生产高价值的香料油。关于S. album适应低温的机制知之甚少。在这项研究中，我们从S. album叶片中获得了100,445,724个原始reads。研究了4℃处理0 ~ 48 h檀香幼苗的生理和转录组学变化。冷胁迫诱导丙二醛、脯氨酸和可溶性碳水化合物积累，增加抗氧化剂水平。共有4424个差异表达基因对寒冷有反应，包括3075个冷诱导基因和1349个冷抑制基因。随着冷胁迫时间的延长，编码转运体、刺激和胁迫反应、防御反应调控的冷应答基因以及所有植物激素信号转导相关基因的表达均有所增加。鉴定了萜类生物合成途径的候选基因，其中8个基因显著参与冷胁迫反应。qRT-PCR基因表达分析显示，在低温胁迫12 h和24 h后，SaCBF2的积累量分别达到对照叶片和根的4、50倍。脑血流依赖通路可能在增强耐寒性中起关键作用。
Santalum album L. (Indian sandalwood) is an economically important plant species because of its ability to produce highly valued perfume oils. Little is known about the mechanisms by which S. album adapts to low temperatures. In this study, we obtained 100,445,724 raw reads by paired-end sequencing from S. album leaves. Physiological and transcriptomic changes in sandalwood seedlings exposed to 4 °C for 0–48 h were characterized. Cold stress induced the accumulation of malondialdehyde, proline and soluble carbohydrates, and increased the levels of antioxidants. A total of 4,424 differentially expressed genes were responsive to cold, including 3,075 cold-induced and 1,349 cold-repressed genes. When cold stress was prolonged, there was an increase in the expression of cold-responsive genes coding for transporters, responses to stimuli and stress, regulation of defense response, as well as genes related to signal transduction of all phytohormones. Candidate genes in the terpenoid biosynthetic pathway were identified, eight of which were significantly involved in the cold stress response. Gene expression analyses using qRT-PCR showed a peak in the accumulation of SaCBF2 to 4, 50-fold more than control leaves and roots following 12 h and 24 h of cold stress, respectively. The CBF-dependent pathway may play a crucial role in increasing cold tolerance.