Label-free immunosensor for the detection of kanamycin using Ag@Fe₃O₄ nanoparticles and thionine mixed graphene sheet.
Label-free immunosensor for the detection of kanamycin using Ag@Fe₃O₄ nanoparticles and thionine mixed graphene sheet.
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DOI：
10.1016/j.bios.2013.04.025
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发表时间：
2013-10
影响因子：
12.6
通讯作者：
Shujun Yu;Q. Wei;B. Du;Dan Wu;He Li;Liang-guo Yan;Hongmin Ma;Yong Zhang
中科院分区：
文献类型：
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作者：
Shujun Yu;Q. Wei;B. Du;Dan Wu;He Li;Liang-guo Yan;Hongmin Ma;Yong Zhang
使用银杂化介孔四氧化三铁纳米粒子（Ag@Fe3O4NPs）和硫堇混合石墨烯片（TH-GS）开发了一种用于检测卡那霉素的高灵敏度无标记免疫传感器。 TH 用作电子转移介体。由于GS良好的电子转移能力，电信号在GS的存在下得到了极大的改善。 Ag@Fe3O4NPs具有比表面积大、导电性好的优点，可以固定更多的卡那霉素抗体并促进电子转移。采用循环伏安法和方波伏安法表征卡那霉素的识别。该免疫传感器在卡那霉素检测中表现出检测限低（15pgmL−1）、线性范围宽（0.050至16ngmL−1）、分析时间短（3min）、稳定性高、选择性好等良好性能。该免疫传感器对猪肉样品进行了评估，收到了满意的结果。
A highly sensitive label-free immunosensor for the detection of kanamycin had been developed using silver hybridized mesoporous ferroferric oxide nanoparticles (Ag@Fe3O4NPs) and thionine mixed graphene sheet (TH-GS). TH was used as an electron transfer mediator. The electrical signal was greatly improved in the presence of GS due to its good electron-transfer ability. With the advantages of large specific surface area and excellent electrical conductivity, Ag@Fe3O4NPs could immobilize more antibodies of kanamycin and promote the electron transfer. Cyclic voltammetry and square wave voltammetry were used to characterize the recognition of kanamycin. The proposed immunosensor showed good performances such as low detection limit (15pgmL−1), wide linear range (from 0.050 to 16ngmL−1), short analysis time (3min), high stability, and good selectivity in the detection of kanamycin. The immunosensor was evaluated for pork meat sample, receiving satisfactory results.