Protective effect of extract of Acanthopanax senticosus harms on dopaminergic neurons in Parkinson's disease mice
Protective effect of extract of Acanthopanax senticosus harms on dopaminergic neurons in Parkinson's disease mice
复制标题
刺五加提取物对帕金森病小鼠多巴胺能神经元损伤的保护作用
DOI：
10.1016/j.phymed.2012.02.006
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发表时间：
2012-05-15
期刊：
影响因子：
7.9
通讯作者：
Lu, Fang
中科院分区：
文献类型：
--
作者：
Liu, Shu-min;Li, Xu-zhao;Lu, Fang
To study the neuroprotective effect of extract of Acanthopanax senticosus Harms against MPTP-induced mice model of Parkinson's disease and its mechanism. The Parkinson's disease mice model was induced by 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine Hydrochloride (MPTP-HCl, 30 mg/kg daily for 5 days). High dose group and low dose group were medicated with extract of Acanthopanax senticosus Harms for 20 days, dose amounted to 182 mg/kg and 45.5 mg/kg daily respectively. The behavioral testing of mice was assessed using pole-climbing test. The levels of Dopamine (DA) and Homovanillic acid (HVA) in striatum were determined by Ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-ToF-MS). The levels of dopamine receptor 1 and 2 in striatum were assayed simultaneously with the help of immunohistochemical method. The level of Caspase-3 protein in substantia nigra was analyzed by Western Blot. From Day 5 during the administration of extract of Acanthopanax senticosus Harms, pole-climbing time in low and high dose group were significantly less than model group (p < 0.05). Compared with model group, the DA level of striatum in low dose group was significantly higher (p < 0.01), the number of dopamine receptor 1 and dopamine receptor 2-positive cells in low and high dose group were significantly less (p < 0.05), the Caspase-3 protein level of substantia nigra in low and high dose group were significantly less (p < 0.05). The neuroprotective effect of extract of Acanthopanax senticosus Harms may be able to protect C57BL/6 mice against MPTP-induced dopaminergic neuronal damage. (C) 2012 Elsevier GmbH. All rights reserved.
